间隔寡核苷酸分型（spoligotyping）和IS6110限制性片段长度多态性分析（IS6110-RFLP）在评估印度结核分枝杆菌基因多样性中的作用

Role of spoligotyping and IS6110-RFLP in assessing genetic diversity of Mycobacterium tuberculosis in India.

作者信息

Mathuria Jitendra Prasad, Sharma Pragya, Prakash Pradyot, Samaria Jai Kumar, Katoch Vishwa Mohan, Anupurba Shampa

机构信息

Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.

出版信息

Infect Genet Evol. 2008 May;8(3):346-51. doi: 10.1016/j.meegid.2008.02.005. Epub 2008 Feb 17.

DOI:10.1016/j.meegid.2008.02.005

PMID:18372222

Abstract

In the present study, genetic diversity analysis of Mycobacterium tuberculosis isolated from patients attending a tertiary care hospital, North India, has been attempted. Eighty three isolates of M. tuberculosis were subjected to DNA fingerprinting using spoligotyping and IS6110-RFLP techniques. Spoligotype patterns showed that central Asian (32.5%), ill defined T (13.2%) and Beijing (10.8%) families were predominant in ongoing transmission of the bacterium. Two STs; ST26 (CAS_Delhi) and ST1 (Beijing) represented 36.1% of the total M. tuberculosis population in eastern Uttar Pradesh, North India. IS6110 RFLP analysis showed that isolates having low and zero copy number of the IS element were 15.6% and 19.2%, respectively. Out of the 47 isolates clustered by spoligotyping, 40 could be further differentiated as unique strains by IS6110-RFLP. Therefore, this study recommends that both the techniques be used simultaneously for DNA fingerprinting of M. tuberculosis in India.

摘要

在本研究中，尝试对从印度北部一家三级护理医院的患者中分离出的结核分枝杆菌进行遗传多样性分析。使用间隔寡核苷酸分型（spoligotyping）和插入序列6110-限制性片段长度多态性（IS6110-RFLP）技术对83株结核分枝杆菌进行了DNA指纹分析。间隔寡核苷酸分型模式显示，中亚家族（32.5%）、不确定的T家族（13.2%）和北京家族（10.8%）在该细菌的持续传播中占主导地位。两个序列型（STs）；ST26（CAS_德里）和ST1（北京）占印度北部北方邦东部结核分枝杆菌总数的36.1%。IS6110 RFLP分析表明，IS元件拷贝数低和为零的分离株分别占15.6%和19.2%。在通过间隔寡核苷酸分型聚类的47株分离株中，40株可通过IS6110-RFLP进一步区分为独特菌株。因此，本研究建议在印度同时使用这两种技术对结核分枝杆菌进行DNA指纹分析。