Jenkins Mark C, O'Brien Celia N, Trout James M
Animal Parasitic Diseases Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA.
J Parasitol. 2008 Feb;94(1):94-8. doi: 10.1645/GE-1313.1.
Monoclonal antibodies (MAb) were prepared against the 40-kDa capsid protein of Cryptosporidium parvum virus (CPV) by immunizing mice with purified recombinant CPV40 protein. In immunoblotting analysis, MAbCPV40-1 bound to a 40-kDa protein in extracts of C. parvum oocysts. This 40-kDa protein was localized in the sporozoite cytoplasm by immunofluorescence (IFA) staining with MAbCPV40-1. In a dot-blot assay, MAbCPV40-1 was capable of detecting 10(2) non-bleach-treated and 10(2)-10(3) bleach-treated C. parvum oocysts. MAbCPV40-1 was capable of detecting CPV40 antigen in both soluble and total C. parvum oocyst protein extracts, indicating a potential use for detecting this parasite in environmental samples.
通过用纯化的重组隐孢子虫病毒（CPV）40 kDa衣壳蛋白免疫小鼠，制备了针对微小隐孢子虫病毒（CPV）40 kDa衣壳蛋白的单克隆抗体（MAb）。在免疫印迹分析中，MAbCPV40 - 1与微小隐孢子虫卵囊提取物中的一种40 kDa蛋白结合。通过用MAbCPV40 - 1进行免疫荧光（IFA）染色，这种40 kDa蛋白定位于子孢子细胞质中。在斑点印迹试验中，MAbCPV40 - 1能够检测到10²个未经漂白处理的以及10² - 10³个经漂白处理的微小隐孢子虫卵囊。MAbCPV40 - 1能够在微小隐孢子虫卵囊的可溶性和总蛋白提取物中检测到CPV40抗原，这表明其在检测环境样品中的这种寄生虫方面具有潜在用途。
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