Al-Abdullah I H, Bentsi-Barnes K, Valiente L, Omori K, Iglesias I, Orr C, Umeadi C, Ferreri K, Todorov I, Al-Sayed M, Kandeel F
City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA.
Transplant Proc. 2008 Mar;40(2):390-2. doi: 10.1016/j.transproceed.2008.01.035.
Pancreas preservation using an oxygenated two-layer method (TLM) has been reported to improve islet yields, as has supplementation of Liberase with Pefabloc. We hypothesized that using both TLM and Pefabloc could enhance islet yield as compared with preservation in University of Wisconsin (UW) or Histidine-Tryptophan Ketoglutarate (HTK) solution.
Ninety-eight pancreata with no significant differences of age, body mass index, or cold ischemia time preserved randomly with UW (n = 40), TLM (n = 48), or HTK (n = 10) were processed with (n = 36) or without (n = 66) Pefabloc.
The total islet equivalent (IEQ) from TLM-preserved pancreata processed with Pefabloc (n = 12) showed lower yields versus those processed without Pefabloc (n = 36): 216,120 +/- 27,906 vs. 301,427 +/- 21,447 IEQ (P < .05). Islets from 1 of 12 (8.33%) pancreata processed with Pefabloc in TLM were transplanted, in contrast with 15/36 TLM (41.67%) pancreata processed without it. Islet yields were not significantly different among pancreata preserved in UW and processed with Pefabloc (n = 17) versus without Pefabloc (n = 23): 342,693 +/- 45,588 versus 266,609 +/- 29,006 IEQ (P = .149). The number of transplants from UW-preserved pancreata was 3/17 (17.65%) when processed with Pefabloc and 4/23 (17.39%) without. Among the HTK group, there was no significant difference in islet yields between pancreata processed with (n = 7) versus without Pefabloc (n = 3): 248,227 +/- 65,294 versus 483,555 +/- 144,070 IEQ (P = .118).
Pefabloc showed no benefit to improve islet yields. Pancreata preserved in TLM provided better transplant quality islets when processed in the absence of Pefabloc.
据报道，采用充氧双层法（TLM）保存胰腺可提高胰岛产量，用抑肽酶补充 Liberase 也有同样效果。我们推测，与在威斯康星大学（UW）溶液或组氨酸 - 色氨酸 - 酮戊二酸（HTK）溶液中保存相比，同时使用 TLM 和抑肽酶可提高胰岛产量。
98 个胰腺，其年龄、体重指数或冷缺血时间无显著差异，随机用 UW（n = 40）、TLM（n = 48）或 HTK（n = 10）保存，分别在有（n = 36）或无（n = 66）抑肽酶的情况下进行处理。
用抑肽酶处理的 TLM 保存胰腺的总胰岛当量（IEQ）（n = 12）产量低于未用抑肽酶处理的（n = 36）：216,120 ± 27,906 与 301,427 ± 21,447 IEQ（P <.05）。在 TLM 中用抑肽酶处理的 12 个胰腺中有 1 个（8.33%）的胰岛进行了移植，相比之下，未用抑肽酶处理的 36 个 TLM 胰腺中有 15 个（41.67%）进行了移植。UW 保存且用抑肽酶处理的胰腺（n = 17）与未用抑肽酶处理的（n = 23）之间的胰岛产量无显著差异：342,693 ± 45,588 与 266,609 ± 29,006 IEQ（P =.149）。UW 保存的胰腺用抑肽酶处理时移植数量为 3/17（17.65%），未用抑肽酶处理时为 4/23（17.39%）。在 HTK 组中，用抑肽酶处理的胰腺（n = 7）与未用抑肽酶处理的（n = 3）之间的胰岛产量无显著差异：248,227 ± 65,294 与 483,555 ± 144,070 IEQ（P =.118）。
抑肽酶对提高胰岛产量无益处。在无抑肽酶处理的情况下，用 TLM 保存的胰腺可提供质量更好的可移植胰岛。
Zotero 插件