精胺和肌胺对非洲爪蟾卵母细胞中表达的N-甲基-D-天冬氨酸受体反应的相反作用。

Opposite effects of spermine and arcaine on responses of N-methyl-D-aspartate receptors expressed in Xenopus oocytes.

作者信息

Maciver C R, Bednar D L, Karbon E W

机构信息

Nova Pharmaceutical Corporation, Baltimore, MD 21224-6522.

出版信息

Neurosci Lett. 1991 Nov 11;132(2):146-50. doi: 10.1016/0304-3940(91)90288-5.

DOI:10.1016/0304-3940(91)90288-5

PMID:1838407

Abstract

The ability of polyamines to modulate N-methyl-D-aspartate (NMDA) receptor function was investigated in Xenopus oocytes injected with rat brain mRNA. Whereas spermine and spermidine augmented NMDA/glycine-induced inwards currents, arcaine (1,4-diguanidinobutane) and 1,10-diaminodecane inhibited the response. The potency of arcaine to inhibit NMDA/glycine-induced currents was unaffected by spermine; similarly, arcaine did not influence the potency of spermine, but did reduce the maximal response to spermine. These findings demonstrate that polyamines exert both positive and negative modulatory control of the NMDA receptor expressed in Xenopus oocytes, and suggest that spermine and arcaine act at distinct sites in the NMDA receptor complex.

摘要

在注射了大鼠脑mRNA的非洲爪蟾卵母细胞中研究了多胺调节N-甲基-D-天冬氨酸（NMDA）受体功能的能力。精胺和亚精胺增强了NMDA/甘氨酸诱导的内向电流，而胍丁胺（1,4-二胍基丁烷）和1,10-二氨基癸烷则抑制了该反应。胍丁胺抑制NMDA/甘氨酸诱导电流的效力不受精胺影响；同样，胍丁胺不影响精胺的效力，但确实降低了对精胺的最大反应。这些发现表明，多胺对非洲爪蟾卵母细胞中表达的NMDA受体发挥正向和负向调节控制作用，并表明精胺和胍丁胺作用于NMDA受体复合物的不同位点。