烟草叶片衰老过程中程序性细胞死亡的原位检测。
In situ detection of programmed cell death in Nicotiana tabacum leaves during senescence.
作者信息
Uzelac B, Janosević D, Budimir S
机构信息
Institute for Biological Research Sinisa Stanković, Bulevar despota Stefana 142, Belgrade, Serbia.
出版信息
J Microsc. 2008 Apr;230(Pt 1):1-3. doi: 10.1111/j.1365-2818.2008.01947.x.
Leaf senescence is a highly regulated developmental process, during which cell constituents are dismantled in an ordered progression. The final death phase of senescence is a type of programmed cell death (PCD). We showed DNA fragmentation characteristic for PCD by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay not only in senescing leaves but also in young, still developing tobacco leaves.
叶片衰老 是一个受到高度调控的发育过程，在此过程中细胞成分按有序进程被分解。衰老的最终死亡阶段是一种程序性细胞死亡（PCD）。我们通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）分析表明，不仅在衰老叶片中，而且在仍处于发育阶段的幼嫩烟草叶片中，都存在PCD特有的DNA片段化现象。
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