全反式维甲酸增强自杀基因疗法对雄激素非反应性前列腺癌的旁观者效应
[All-trans retinoic acid enhances bystander effect of suicide-gene therapy against androgen-unresponsive prostate cancer].
作者信息
Chen Wei-Guo, Yan Chun-Yin, Hou Jian-Quan, Wen Duan-Gai, Pu Jin-Xian, Wang Heng-Bing
机构信息
Department of Urology, First Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215006, China.
出版信息
Zhonghua Nan Ke Xue. 2008 Feb;14(2):122-5.
OBJECTIVE
To investigate the enhancing effect of all-trans retinoic acid (ATRA) on the bystander effect of the herpes simplex virus thymidine kinase(HSV-TK)/ganciclovir (GCV) against androgen unresponsive prostate cancer.
METHODS
The bystander effect of the HSV-TK/GCV system was measured by methyl thiazolyl tetrazolium (MTT) assay on PC-3 cells before and after ATRA treatment. The growth and the histopathology of transplant tumors were observed in 4 groups of nude mice with prostate cancer.
RESULTS
ATRA augmented significantly the bystander effect of the HSV-TK/GCV system by reducing TK positive PC-3 cells from 50% to 30% (P < 0.05). HSV-TK showed an inhibiting effect, while ATRA with the HSV-TK/GCV system produced significant effect on prostate cancer 1 week earlier than the former (P < 0.05).
CONCLUSION
ATRA can argument the in vivo and in vitro bystander effect of the HSV-TK/GCV system in the treatment of androgen unresponsive prostate cancer.
目的
探讨全反式维甲酸（ATRA）对单纯疱疹病毒胸苷激酶（HSV-TK）/更昔洛韦（GCV）抗雄激素非依赖性前列腺癌旁观者效应的增强作用。
方法
采用甲基噻唑基四氮唑（MTT）法检测ATRA处理前后PC-3细胞中HSV-TK/GCV系统的旁观者效应。观察4组前列腺癌裸鼠移植瘤的生长及组织病理学变化。
结果
ATRA通过将TK阳性PC-3细胞从50%降至30%，显著增强了HSV-TK/GCV系统的旁观者效应（P<0.05）。HSV-TK显示出抑制作用，而ATRA与HSV-TK/GCV系统联合使用比单独使用HSV-TK对前列腺癌产生显著作用的时间提前1周（P<0.05）。
结论
ATRA可增强HSV-TK/GCV系统在体内外对雄激素非依赖性前列腺癌的旁观者效应。
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