Lin Yan-jun, Liao Zhi-min, Zhang Jin-feng, Zhang Lan
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Jan;39(1):108-10, 125.
To reveal the mechanism of propofol aortic infusion in protecting ischemia-reperfusion spinal cords through detecting the IL-6 levels in the spinal cords and observing the neurological functioning.
Fifty six healthy New Zealand White rabbits were randomly allocated into three groups, 4 in the control group, 26 in the Saline infusion group and 26 in the Propofol infusion group. The spinal cord ischemia was induced by infrarenal aorta occlusion for 30 minutes. Propofol were infused through aorta distal to the occlude sites of the rabbits in the Propofol infusion group continuously with a pump at a rate of 50 mg/kg x 30 min. The same volume of Saline were infused in the same way and at the same rate to the rabbits in the Saline infusion group. The lumbar segments of 4-6 spinal cords were harvested and the IL-6 were examined 0 hour or 2 hours after reperfusion. The spinal cords of the rabbits in the control group were harvested right after anesthesia. Forty eight hours after reperfusion, the neurological functioning of the rabbits was assessed with the Tarlov scale system and the normal motor neurons of anterior horn of the lumbar segments of 4-6 spinal cords were counted.
The IL-6 levels of the rabbits in the Saline infusion group were significant higher than those in the control group and the Propofol infusion group (P < 0.05). There was no significant difference in the IL-6 level between the Propofol infusion group and the control group (P > 0.05). A significant increase of IL-6 in the rabbits in the Saline infusion group and the Propofol infusion group 2 hours after reperfusion was observed (P < 0.05). The rabbits in the Propofol infusion group had less paraplegia (30%) and more normal neurons (8.4) than those in the Saline infusion group (80% and 1.9, respectively) (P < 0.05).
Occlusion of aorta increases IL-6 in the injured spinal cords. Propofol aortic infusion can decrease the IL-6 level and improve the neurological functioning, which is perhaps associated with the inflammatory inhibition effect of Propofol.
通过检测脊髓中白细胞介素-6（IL-6）水平并观察神经功能，揭示丙泊酚主动脉输注对缺血再灌注脊髓的保护机制。
56只健康新西兰白兔随机分为三组，对照组4只，生理盐水输注组26只，丙泊酚输注组26只。通过肾下腹主动脉阻断30分钟诱导脊髓缺血。丙泊酚输注组的兔子在阻断部位远端的主动脉处用泵以50mg/kg×30分钟的速率持续输注丙泊酚。以相同方式和相同速率向生理盐水输注组的兔子输注相同体积的生理盐水。在再灌注后0小时或2小时采集4 - 6节段脊髓的腰段并检测IL-6。对照组兔子在麻醉后立即采集脊髓。再灌注48小时后，用Tarlov评分系统评估兔子的神经功能，并计数4 - 6节段脊髓腰段前角的正常运动神经元。
生理盐水输注组兔子的IL-6水平显著高于对照组和丙泊酚输注组（P < 0.05）。丙泊酚输注组和对照组之间的IL-6水平无显著差异（P > 0.05）。观察到生理盐水输注组和丙泊酚输注组兔子在再灌注后2小时IL-6显著增加（P < 0.05）。丙泊酚输注组的兔子截瘫发生率（30%）低于生理盐水输注组（80%），正常神经元数量（8.4个）多于生理盐水输注组（分别为1.9个）（P < 0.05）。
主动脉阻断会增加损伤脊髓中的IL-6。丙泊酚主动脉输注可降低IL-6水平并改善神经功能，这可能与丙泊酚的抗炎作用有关。
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