聚阳离子纳米结构脂质载体，一种用三油酸甘油酯构建的新型非病毒载体，用于高效基因递送。

Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery.

作者信息

Zhang Zhiwen, Sha Xianyi, Shen Anle, Wang Yongzhong, Sun Zhaogui, Gu Zheng, Fang Xiaoling

机构信息

School of Pharmacy, Fudan University, Lane 138, Yi Xue Yuan Road, Shanghai 200032, China.

出版信息

Biochem Biophys Res Commun. 2008 Jun 6;370(3):478-82. doi: 10.1016/j.bbrc.2008.03.127. Epub 2008 Apr 3.

DOI:10.1016/j.bbrc.2008.03.127

PMID:18395002

Abstract

A novel nonviral gene transfer vector was developed by modifying nanostructured lipid carrier (NLC) with cetylated polyethylenimine (PEI). Polycation nanostructured lipid carrier (PNLC) was prepared using the emulsion-solvent evaporation method. Its in vitro gene transfer properties were evaluated in the human lung adenocarcinoma cell line SPC-A1 and Chinese Hamster Ovary (CHO) cells. Enhanced transfection efficiency of PNLC was observed after the addition of triolein to the PNLC formulation and the transfection efficiency of the optimized PNLC was comparable to that of Lipofectamine 2000. In the presence of 10% serum the transfection efficiency of the optimal PNLC was not significantly changed in either cell line, whereas that of Lipofectamine 2000 was greatly decreased in both. Thus, PNLC is an effective nonviral gene transfer vector and the gene delivery activity of PNLC was enhanced after triolein was included into the PNLC formulation.

摘要

通过用十六烷基化聚乙烯亚胺（PEI）修饰纳米结构脂质载体（NLC），开发了一种新型非病毒基因转移载体。采用乳液-溶剂蒸发法制备了聚阳离子纳米结构脂质载体（PNLC）。在人肺腺癌细胞系SPC-A1和中国仓鼠卵巢（CHO）细胞中评估了其体外基因转移特性。在PNLC制剂中加入三油酸甘油酯后，观察到PNLC的转染效率提高，优化后的PNLC的转染效率与Lipofectamine 2000相当。在10%血清存在的情况下，两种细胞系中最佳PNLC的转染效率均无明显变化，而Lipofectamine 2000的转染效率在两种细胞系中均大幅下降。因此，PNLC是一种有效的非病毒基因转移载体，将三油酸甘油酯加入PNLC制剂后，PNLC的基因递送活性增强。