表达绿色荧光蛋白（GFP）与β-半乳糖苷酶核定位融合蛋白的Cre报告基因小鼠揭示了表达Pax3的前体细胞的新衍生物。
Cre reporter mouse expressing a nuclear localized fusion of GFP and beta-galactosidase reveals new derivatives of Pax3-expressing precursors.
作者信息
Stoller Jason Z, Degenhardt Karl R, Huang Li, Zhou Diane D, Lu Min Min, Epstein Jonathan A
出版信息
Genesis. 2008 Apr;46(4):200-4. doi: 10.1002/dvg.20384.
Abstract
A new Cre-reporter strain of mouse has been developed that expresses a fusion protein derived from the lacZ gene fused to GFP with a nuclear localization signal. This construct is expressed from the ROSA26 locus upon Cre-mediated recombination that removes a loxP-flanked PGK-neo cassette, thus allowing for detection of Cre activity in all tissues. This reporter strain, which is similar to prior R26R and R26EGFP strains, has certain advantages related to the nuclear expression and the combined expression of both beta-galactosidase and GFP activities. We show that the use of this newly developed reporter line allows for enhanced resolution, detection and co-localization. Thus, we report a previously unrecognized subset of venous endothelial cells derived from Pax3 expressing precursors.
摘要
一种新的小鼠Cre报告基因品系已经构建成功，该品系表达一种融合蛋白，该融合蛋白由与带有核定位信号的绿色荧光蛋白（GFP）融合的乳糖酶基因（lacZ）衍生而来。在Cre介导的重组作用下，该构建体从ROSA26位点表达，去除了两侧带有loxP的PGK-新霉素盒，从而能够检测所有组织中的Cre活性。该报告基因品系与先前的R26R和R26EGFP品系相似，在核表达以及β-半乳糖苷酶和GFP活性的联合表达方面具有一定优势。我们表明，使用这种新开发的报告基因系可提高分辨率、检测能力和共定位能力。因此，我们报告了一个以前未被认识的静脉内皮细胞亚群，其来源于表达Pax3的前体细胞。
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本文引用的文献
1
Abnormal venous and arterial patterning in Chordin mutants.
Dev Dyn. 2007 Sep;236(9):2586-93. doi: 10.1002/dvdy.21287.
2
Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.
Eur J Immunol. 2007 Jan;37(1):43-53. doi: 10.1002/eji.200636745.
3
Smooth muscle of the dorsal aorta shares a common clonal origin with skeletal muscle of the myotome.
Development. 2006 Feb;133(4):737-49. doi: 10.1242/dev.02226.
4
Optimized beta-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos.
Genesis. 2006 Feb;44(2):57-65. doi: 10.1002/gene.20186.
5
Insertion of Cre into the Pax3 locus creates a new allele of Splotch and identifies unexpected Pax3 derivatives.
Dev Biol. 2005 Apr 15;280(2):396-406. doi: 10.1016/j.ydbio.2005.02.002.
6
The neural crest is contiguous with the cardiac conduction system in the mouse embryo: a role in induction?
Anat Embryol (Berl). 2004 Aug;208(5):389-93. doi: 10.1007/s00429-004-0401-6. Epub 2004 Jul 10.
7
BMP receptor IA is required in mammalian neural crest cells for development of the cardiac outflow tract and ventricular myocardium.
Development. 2004 May;131(9):2205-18. doi: 10.1242/dev.01086. Epub 2004 Apr 8.
8
Identification of minimal enhancer elements sufficient for Pax3 expression in neural crest and implication of Tead2 as a regulator of Pax3.
Development. 2004 Feb;131(4):829-37. doi: 10.1242/dev.00975. Epub 2004 Jan 21.
9
PlexinA2 and semaphorin signaling during cardiac neural crest development.
Development. 2001 Aug;128(16):3071-80. doi: 10.1242/dev.128.16.3071.
10
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