脂质双分子层中多不饱和烃链的结构与动力学——对G蛋白偶联受体功能的意义
Structure and dynamics of polyunsaturated hydrocarbon chains in lipid bilayers-significance for GPCR function.
作者信息
Gawrisch Klaus, Soubias Olivier
机构信息
Section of NMR, Laboratory of Membrane Biochemistry and Biophysics, NIAAA, NIH, Bethesda, MD 20892, United States.
出版信息
Chem Phys Lipids. 2008 May;153(1):64-75. doi: 10.1016/j.chemphyslip.2008.02.016. Epub 2008 Mar 13.
Abstract
This review summarizes results of our recent solid-state NMR investigations on polyunsaturated 18:0-22:6n3-PC/PE/PS and 18:0-22:5n6-PC bilayers. Data on structure and dynamics of the polyunsaturated docosahexaenoyl (DHAn3, 22:6n3) and docosapentaenoyl chains (DPAn6, 22:5n6), investigated at physiological conditions, are reported. Lipid-protein interaction was studied on reconstituted bilayers containing the G-protein coupled membrane receptor (GPCR) rhodopsin as well as on rod outer segment (ROS) disk membranes prepared from bovine retinas. Results reveal surprisingly rapid conformational transitions of polyunsaturated chains and existence of weakly specific interactions of DHAn3 with spatially distinct sites on rhodopsin.
摘要
本综述总结了我们最近对多不饱和18:0 - 22:6n3 - PC/PE/PS和18:0 - 22:5n6 - PC双层膜进行的固态核磁共振研究结果。报告了在生理条件下对多不饱和二十二碳六烯酰基链（DHA n3，22:6n3）和二十二碳五烯酰基链（DPA n6，22:5n6）的结构和动力学研究数据。研究了含G蛋白偶联膜受体（GPCR）视紫红质的重组双层膜以及从牛视网膜制备的视杆外段（ROS）盘膜上的脂-蛋白相互作用。结果显示，多不饱和链具有惊人的快速构象转变，并且DHA n3与视紫红质上空间不同位点存在弱特异性相互作用。
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