Geffroy-Luseau Alexandrine, Jégo Gaëtan, Bataille Régis, Campion Loïc, Pellat-Deceunynck Catherine
INSERM U601, Department of Cancer Research, Biology Institute, 9 Quai Moncousu, F-44000 Nantes, France.
Int Immunol. 2008 Jun;20(6):775-82. doi: 10.1093/intimm/dxn035. Epub 2008 Apr 8.
The aim of this in vitro study was to evaluate if osteoclasts (OCs) and dendritic cells (DCs), both of monocyte origin, can support the survival of normal human plasma cells (PCs). PCs differentiate from plasmablasts (PBs) arising from activated B cells, essentially memory B cells. To study the survival of both PBs (CD20(low)CD38(high)CD138(neg)) and PCs (CD20(neg)CD38(bright)CD138(bright)), we generated pre-PBs (CD20(low)CD38(pos)CD138(neg)) from CD40-activated B cells (CD20(high)CD38(neg)CD138(neg)) and cultured them on DCs or OCs in the presence of added IL-6. By quantitative and qualitative study, we showed that DCs support the survival of PBs and early PCs, but not that of PCs. In contrast, OCs support the survival of PBs, early PCs and PCs. PCs surviving on OCs 12 days after pre-PB input display phenotypic features of bone marrow PCs, CD138(bright)CD38(bright)HLA-DR(low)CD45(dim). The ability for OCs to support the survival of PCs was fully dependent on cell-cell contact and not inhibited by BCMA-Fc suggesting that secreted BAFF and APRIL were not involved.
本体外研究的目的是评估源自单核细胞的破骨细胞（OCs）和树突状细胞（DCs）是否能够支持正常人浆细胞（PCs）的存活。PCs由活化B细胞（主要是记忆B细胞）产生的成浆细胞（PBs）分化而来。为了研究PBs（CD20(low)CD38(high)CD138(neg)）和PCs（CD20(neg)CD38(bright)CD138(bright)）的存活情况，我们从CD40活化的B细胞（CD20(high)CD38(neg)CD138(neg)）中生成前PBs（CD20(low)CD38(pos)CD138(neg)），并在添加白细胞介素-6的情况下将它们培养于DCs或OCs上。通过定量和定性研究，我们发现DCs支持PBs和早期PCs的存活，但不支持PCs的存活。相比之下，OCs支持PBs、早期PCs和PCs的存活。在前PB输入12天后在OCs上存活的PCs表现出骨髓PCs的表型特征，即CD138(bright)CD38(bright)HLA-DR(low)CD45(dim)。OCs支持PCs存活的能力完全依赖于细胞间接触，且不受BCMA-Fc的抑制，这表明分泌的BAFF和APRIL未参与其中。
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