[山东省慢性肾小球肾炎与人类白细胞抗原关系的研究]

Zhao Jing-jie, Liu Jun-li, Zhang Cai

Laboratory of Clinical Molecular Biology, Second Hospital of Shandong University, Jinan, China.

Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Oct;28(10):1030-5.

OBJECTIVE

The purpose of this study was to observe the association between chronic glomerulonephritis (CGN) and human leukocyte antigen (HLA) on DNA level in order to identify susceptible and protective genes and to further explain the possible pathogenesis of CGN.

METHODS

1073 renal transplantation patients with Han ethnicity were included in this study. All patients were recruited from three provincial Hospitals during the past ten years. The control group contained 7418 healthy Han volunteer donors from Shandong Hematopoietic Stem Cell Data Bank of China. We collected data about the polymorphism of HLA-I , II and DRB1. Gene frequency (GF), relative risk (RR) and correlation test were analyzed using statistical software. Some patients carrying the susceptible genes were followed up for 1,3 and 5 years, and compared their survival rate respectively.

RESULTS

The frequency of HLA-A23, A25,B15, B40, B53 and DRB1 * 18 alleles increased significantly in CGN patients than in controls, showing that they might be the suspicious susceptibility genes of CGN. After the follow-up periods, the prognosis of patients with the susceptible genes was worse than the controls. The frequency of haplotypes of A23-B44-DRB1 * 18, A25-B15-DRB1 * 07, A3-B70-DRB1 * 11, A68-B13-DRB1 * 04, A11-B10-DRB1 * 12 increased significantly in CGN patients than in controls. There were 8 lower frequencies alleles (including A20, A22, A35, A36, A38, B21, B73 and B78) in CGN patients that were not found in the control group. The frequencies of the HLA-A32, A33, B50, B58, B60, B71, DRB1 * 16 alleles decreased significantly in CGN patients than in controls, showing that they might be the protective genes of CGN.

CONCLUSION

Our data showed that there might be corresponding susceptibility and protective genes of CGN in Han population, in Shandong. There was significant association between the five common haplotype and CGN in Shandong population. However, the prognosis of the patients with the susceptibility genes was worse than the controls.

目的

本研究旨在观察慢性肾小球肾炎（CGN）与人类白细胞抗原（HLA）在DNA水平上的关联，以确定易感基因和保护基因，并进一步解释CGN可能的发病机制。

方法

本研究纳入1073例汉族肾移植患者。所有患者均为过去十年间从三家省级医院招募而来。对照组包含来自中国山东造血干细胞数据库的7418名健康汉族志愿者供者。我们收集了关于HLA - I、II和DRB1多态性的数据。使用统计软件分析基因频率（GF）、相对风险（RR）和相关性检验。对部分携带易感基因的患者进行1年、3年和5年随访，并分别比较其生存率。

结果

CGN患者中HLA - A23、A25、B15、B40、B53和DRB1 * 18等位基因频率显著高于对照组，表明它们可能是CGN的可疑易感基因。随访期后，携带易感基因患者的预后比对照组差。CGN患者中A23 - B44 - DRB1 * 18、A25 - B15 - DRB1 * 07、A3 - B70 - DRB1 * 11、A68 - B13 - DRB1 * 04、A11 - B10 - DRB1 * 12单倍型频率显著高于对照组。CGN患者中有8个低频等位基因（包括A20、A22、A35、A36、A38、B21、B73和B78）在对照组中未发现。CGN患者中HLA - A32、A33、B50、B58、B60、B71、DRB1 * 16等位基因频率显著低于对照组，表明它们可能是CGN的保护基因。