高效薄层色谱法测定胶囊中雷贝拉唑和多潘立酮及其方法验证

HPTLC determination of rabeprazole and domperidone in capsules and its validation.

作者信息

Patel Bhavesh H, Suhagia Bhanubhai N, Patel Madhabhai M, Patel Jignesh R

机构信息

Department of Pharmaceutical Chemistry, S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva, Mehsana-382711, Gujarat, India.

出版信息

J Chromatogr Sci. 2008 Apr;46(4):304-7. doi: 10.1093/chromsci/46.4.304.

DOI:10.1093/chromsci/46.4.304

PMID:18402720

Abstract

This paper describes a validated high-performance thin-layer chromatography (HPTLC) method for simultaneous estimation of rabeprazole (RA) and domperidone (DO) in pure powder and in capsule formulations. An HPTLC method separation is achieved on an aluminum sheet of silica gel 60F(254) using ethyl acetate-methanol-benzene-acetonitrile (30:20:30:20 v/v) as mobile phase. Quantitation is achieved with UV detection at 287 nm over a concentration range of 400-1200 ng/spot and 600-1800 ng/spot with mean recovery of 99.82 +/- 0.74 and 99.43 +/- 0.68 for RA and DO, respectively, in the HPTLC method. This method is simple, precise, and sensitive, and it is applicable for the simultaneous determination of RA and DO in pure powder and in capsule formulation.

摘要

本文描述了一种经过验证的高效薄层色谱（HPTLC）方法，用于同时测定纯粉末和胶囊制剂中雷贝拉唑（RA）和多潘立酮（DO）的含量。使用硅胶60F(254)铝板，以乙酸乙酯 - 甲醇 - 苯 - 乙腈（30:20:30:20 v/v）作为流动相，通过HPTLC方法实现分离。在287 nm处进行紫外检测进行定量，RA的浓度范围为400 - 1200 ng/斑点，DO的浓度范围为600 - 1800 ng/斑点，在HPTLC方法中，RA和DO的平均回收率分别为99.82 +/- 0.74和99.43 +/- 0.68。该方法简单、精确且灵敏，适用于同时测定纯粉末和胶囊制剂中的RA和DO。