Bolkent Sema, Sacan Ozlem, Yanardag Refiye, Bolkent Sehnaz
Department of Medical Biology, Faculty of Cerrahpasa Medicine, Istanbul University, Istanbul, Turkey.
Int J Toxicol. 2008 Mar-Apr;27(2):217-22. doi: 10.1080/10915810801992384.
Cadmium (Cd) is a highly toxic metal. It has an indirect role in the generation of various free radicals. Antioxidants such as vitamin E, vitamin C, and selenium are important for preventing the damage caused by reactive oxygen species. This study was undertaken to examine the effect of acute cadmium and/or antioxidants on serum lipid metabolism, tissue glutathione, and lipid peroxidation (LPO) levels, and ghrelin and metallothionein production in the gastric fundus mucosa of rats. Cd (2 mg/kg/day CdCl(2)) was administered to rats for 8 days, intraperitoneally. Vitamin E (250 mg /kg/day) + vitamin C (250 mg/kg/day) + sodium selenate (0.25 mg /kg/day) were administered to rats orally at the same time. The animals were treated by antioxidants 1 h prior to treatment with Cd every day. Gastric tissue homogenates were used for protein and glutathione and LPO levels. Phospholipid and total lipid levels were determined in serum. Gastric fundus sections examined for histopathological changes and by immunohistochemistry for expression of ghrelin and metallothionein. In the group treated with Cd, degenerative changes such as discontinuity in the surface epithelium were observed. The degenerative changes induced by Cd were decreased in the group given vitamin E + vitamin C + selenium. There was no significant change in ghrelin- and metallothionein-immunoreactive cells in fundus mucosa. Stomach glutathione levels insignificantly decreased in the Cd groups, but in the Cd group given antioxidant, stomach glutathione levels were significantly increased. Serum phospholipid and total lipid levels were significantly increased in the Cd groups. On the other hand, treatment with antioxidants reversed these effects. These results indicate that antioxidants partly prevent the toxicity of Cd in rat gastric fundus.
镉（Cd）是一种剧毒金属。它在各种自由基的产生中起间接作用。维生素E、维生素C和硒等抗氧化剂对于预防活性氧引起的损伤很重要。本研究旨在探讨急性镉和/或抗氧化剂对大鼠胃底黏膜血清脂质代谢、组织谷胱甘肽、脂质过氧化（LPO）水平以及胃饥饿素和金属硫蛋白产生的影响。给大鼠腹腔注射Cd（2 mg/kg/天CdCl₂），持续8天。同时给大鼠口服维生素E（250 mg/kg/天）+维生素C（250 mg/kg/天）+硒酸钠（0.25 mg/kg/天）。每天在给予Cd前1小时用抗氧化剂处理动物。胃组织匀浆用于检测蛋白质、谷胱甘肽和LPO水平。测定血清中的磷脂和总脂质水平。检查胃底切片的组织病理学变化，并通过免疫组织化学检测胃饥饿素和金属硫蛋白的表达。在Cd处理组中，观察到表面上皮连续性中断等退行性变化。在给予维生素E+维生素C+硒的组中，Cd诱导的退行性变化有所减少。胃底黏膜中胃饥饿素和金属硫蛋白免疫反应性细胞无明显变化。Cd组胃组织谷胱甘肽水平略有下降，但在给予抗氧化剂的Cd组中，胃组织谷胱甘肽水平显著升高。Cd组血清磷脂和总脂质水平显著升高。另一方面，抗氧化剂治疗可逆转这些作用。这些结果表明，抗氧化剂可部分预防Cd对大鼠胃底的毒性。
