养肝利胆颗粒对诱导性胆固醇结石豚鼠胆结石形成率及血浆胆囊收缩素含量的影响

Zhang Si-bo, Fang Bang-jiang

Department of Hepatobiliary Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

Zhong Xi Yi Jie He Xue Bao. 2008 Apr;6(4):405-8. doi: 10.3736/jcim20080415.

OBJECTIVE

To explore the effects of Yanggan Lidan Granule (YGLDG), a compound traditional Chinese herbal medicine for nourishing liver and improving choleresis, on the rate of gallstone formation and content of plasma cholecystokinin in guinea pigs with induced cholesterol gallstones.

METHODS

Eighty guinea pigs were randomly divided into 4 groups, which were normal control group, untreated group, YGLDG-treated group and ursodeoxycholic acid (UDCA)-treated group (n=20). Except the normal control group, gallstones were induced by high-cholesterol diet in the guinea pigs. YGLDG (1.81 g/kg daily) and UDCA (30.12 mg/kg daily) were given orally to guinea pigs in the corresponding group respectively for seven weeks; however, the guinea pigs of normal control group and untreated group were administered with normal saline. The physical signs of the guinea pigs and the rates of gallstone formation were examined, and the content of cholecystokinin (CCK) in the plasma was detected by radio-immunoassay.

RESULTS

YGLDG could obviously improve the ethological signs of the guinea pigs. Gallstone formation rate of the untreated group (82.35%) was significantly increased as compared with that of the normal control group (5.26%) (P<0.01), while the gallstone formation rates of the YGLDG-treated group (27.28%) and UDCA-treated group (38.89%) were lower than that of the untreated group (P<0.05). The content of CCK in plasma of the untreated group was significantly lower than that of the normal control group (P<0.01), while CCK content of the YGLDG-treated group and UDCA-treated group was lower than that of the normal control group, but both of them were significantly higher than that of the untreated group (P<0.01). The difference of CCK levels between YGLDG-treated group and UDCA-treated group had no statistical significance (P>0.05).

CONCLUSION

YGLDG can significantly decrease the rate of gallstone formation in guinea pigs. It may be related to elevating the content of CCK in the plasma.

目的

探讨养肝利胆颗粒（YGLDG），一种用于养肝及促进胆汁分泌的复方中药，对诱导性胆固醇结石豚鼠的胆结石形成率及血浆胆囊收缩素含量的影响。

方法

80只豚鼠随机分为4组，即正常对照组、未治疗组、YGLDG治疗组和熊去氧胆酸（UDCA）治疗组（n = 20）。除正常对照组外，豚鼠通过高胆固醇饮食诱导形成胆结石。分别给相应组的豚鼠口服YGLDG（每日1.81 g/kg）和UDCA（每日30.12 mg/kg），持续7周；而正常对照组和未治疗组的豚鼠给予生理盐水。检查豚鼠的体征和胆结石形成率，采用放射免疫法检测血浆中胆囊收缩素（CCK）的含量。

结果

YGLDG能明显改善豚鼠的行为体征。未治疗组的胆结石形成率（82.35%）与正常对照组（5.26%）相比显著升高（P<0.01），而YGLDG治疗组（27.28%）和UDCA治疗组（38.89%）的胆结石形成率低于未治疗组（P<0.05）。未治疗组血浆中CCK的含量显著低于正常对照组（P<0.01），而YGLDG治疗组和UDCA治疗组的CCK含量低于正常对照组，但均显著高于未治疗组（P<0.01）。YGLDG治疗组和UDCA治疗组之间CCK水平的差异无统计学意义（P>0.05）。