Sarais Giorgia, Caboni Pierluigi, Sarritzu Erika, Russo Mariateresa, Cabras Paolo
Department of Toxicology, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy.
J Agric Food Chem. 2008 May 14;56(9):2939-43. doi: 10.1021/jf7037407. Epub 2008 Apr 12.
Neem-based insecticides containing azadirachtin and related azadirachtoids are widely used in agriculture. Here, we report an analytical method for the rapid and accurate quantification of the insecticide azadirachtin A and B and other azadirachtoids such as salannin, nimbin, and their deacetylated analogues on tomatoes and peaches. Azadirachtoids were extracted from fruits and vegetables with acetonitrile. Using high-performance liquid chromatography/electrospray ionization tandem mass spectrometer, azadirachtoids were selectively detected monitoring the multiple reaction transitions of sodium adduct precursor ions. For azadirachtin A, calibration was linear over a working range of 1-1000 microg/L with r > 0.996. The limit of detection and limit of quantification for azadirachtin A were 0.4 and 0.8 microg/kg, respectively. The presence of interfering compounds in the peach and tomato extracts was evaluated and found to be minimal. Because of the linear behavior, it was concluded that the multiple reaction transitions of sodium adduct ions can be used for analytical purposes, that is, for the identification and quantification of azadirachtin A and B and related azadirachtoids in fruit and vegetable extracts at trace levels.
含有印楝素及相关类印楝素的印楝基杀虫剂在农业中被广泛使用。在此，我们报告一种分析方法，用于快速、准确地定量测定番茄和桃子上的杀虫剂印楝素A和B以及其他类印楝素，如萨林宁、印楝宁及其脱乙酰类似物。类印楝素用乙腈从水果和蔬菜中提取。使用高效液相色谱/电喷雾电离串联质谱仪，通过监测钠加合前体离子的多反应跃迁来选择性检测类印楝素。对于印楝素A，在1 - 1000微克/升的工作范围内校准呈线性，r > 0.996。印楝素A的检测限和定量限分别为0.4和0.8微克/千克。对桃子和番茄提取物中干扰化合物的存在情况进行了评估，发现干扰极小。由于呈线性关系，得出结论：钠加合离子的多反应跃迁可用于分析目的，即用于鉴定和定量水果和蔬菜提取物中痕量水平的印楝素A和B以及相关类印楝素。
