Ignatova M, Leguerinel I, Guilbot M, Prévost H, Guillou S
UMR-INRA 1014 SECALIM, ENITIAA, rue de la Géraudière, Nantes, France.
J Appl Microbiol. 2008 Sep;105(3):875-83. doi: 10.1111/j.1365-2672.2008.03812.x. Epub 2008 Apr 9.
Study the effect of redox potential and pH of the heating media on Listeria monocytogenes heat resistance and model its action at fixed temperature.
The heat resistance of Listeria monocytogenes at 58 degrees C was studied in Brain Heart Infusion broth as a function of pH (from 5.0 to 7.0) and redox potential (E(h7)). The media redox was adjusted with nitrogen gas, potassium ferricyanide and dithiothreitol. A Weibull model was used to fit survival curves. The heat resistance parameter (delta(58 degrees C)) was estimated from each inactivation curve. A major effect of pH was observed. Bigelow model was used to describe the effect of redox potential on the apparent L. monocytogenes heat resistance. The highest delta(58 degrees C) values have been obtained at pH 7.0 and oxidizing conditions.
The developed model indicates that the E(h7) has a significant effect and varied depending on the pH of the heating media. The z(redox) values, calculated from delta(58 degrees C) allowed quantifying the influence of heating media redox potential on L. monocytogenes thermal inactivation.
The obtained model shows the action of redox potential on L. monocytogenes thermal destruction and might be useful to take into account in food thermal processes.
研究加热介质的氧化还原电位和pH值对单核细胞增生李斯特菌耐热性的影响，并在固定温度下对其作用进行建模。
在脑心浸液肉汤中研究了58℃时单核细胞增生李斯特菌的耐热性与pH值（5.0至7.0）和氧化还原电位（E(h7)）的关系。通过氮气、铁氰化钾和二硫苏糖醇调节培养基的氧化还原状态。使用韦布尔模型拟合存活曲线。从每条失活曲线估计耐热性参数（δ(58℃)）。观察到pH值有主要影响。使用比奇洛模型描述氧化还原电位对单核细胞增生李斯特菌表观耐热性的影响。在pH 7.0和氧化条件下获得了最高的δ(58℃)值。
所建立的模型表明E(h7)有显著影响，且随加热介质的pH值而变化。根据δ(58℃)计算的z(redox)值能够量化加热介质氧化还原电位对单核细胞增生李斯特菌热失活的影响。
所获得的模型显示了氧化还原电位对单核细胞增生李斯特菌热破坏的作用，在食品热加工过程中考虑这一点可能会有用。
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