扫描隧道显微镜下的平行链DNA：双螺旋的主要特征
Parallel stranded DNA under scanning tunnelling microscope: the main characteristics of the double helix.
作者信息
Tchurikov N A, Zhu J D, Li M Q, Chernov B K
机构信息
Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
出版信息
Nucleic Acids Symp Ser. 1991(24):169-70.
Using the scanning tunnelling microscopy we have directly observed the parallel stranded DNA of 43 bp in length, containing alternating AT-stretches. The double helix is right-handed and has the same width of each grooves equal to 17.4 A. The average pitch of the helical turn is about 34 A. The parallel double helix possesses no more than 8.6 bases per one turn. The diameter of the parallel stranded DNA molecule is 17-18 A. We conclude that in parallel DNA double helix the angle between N-glycoside bounds in trans-Crick-Watson base pairs is close to 180 degrees.
利用扫描隧道显微镜，我们直接观察到了长度为43个碱基对的平行链DNA，其中含有交替的AT序列。双螺旋为右手螺旋，每条沟的宽度相同，均为17.4埃。螺旋圈的平均螺距约为34埃。平行双螺旋每圈不超过8.6个碱基。平行链DNA分子的直径为17 - 18埃。我们得出结论，在平行DNA双螺旋中，反式-克里克-沃森碱基对中N-糖苷键之间的夹角接近180度。
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