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通过三相分配法从米曲霉粗提物中纯化α-半乳糖苷酶和转化酶。

Purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae.

作者信息

Dhananjay S K, Mulimani V H

机构信息

Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India.

出版信息

Biotechnol Lett. 2008 Sep;30(9):1565-9. doi: 10.1007/s10529-008-9722-5. Epub 2008 Apr 15.

Abstract

Alpha-galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and pH). Alpha-galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. The fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by SDS-PAGE. Both purified preparations showed electrophoretic homogeneity on SDS-PAGE.

摘要

在不同条件(硫酸铵、叔丁醇与粗提取物的比例、温度和pH值)下,α-半乳糖苷酶和转化酶在三相分配系统的连贯中间相中积累。α-半乳糖苷酶和转化酶分别纯化了15倍和12倍,活性回收率分别为50%和54%。通过SDS-PAGE分析了三相分配产生的界面沉淀物的组分。两种纯化制剂在SDS-PAGE上均显示出电泳均一性。

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