Gong Jin, Lao Xue-Jun, Wang Xi-Mo, Long Gang, Jiang Tao, Chen Shi
Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
World J Gastroenterol. 2008 Apr 21;14(15):2338-42. doi: 10.3748/wjg.14.2338.
To compare the preservation of non-heart-beating donor (NHBD) livers in cold histidine-trytophan-ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP).
Livers harvested from healthy pigs were stored for 10 h in cold HTK solution (group A, n = 4) or perfused with oxygenated autologous blood at body temperature (group B, n = 4). Both groups were then tested on the circuit for 4 h. Bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of extracorporeal livers were tested in each group. Liver tissues from each group were examined at the end of reperfusion.
At 1, 2, 3 and 4 h after reperfusion, bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of livers in group A were statistically different from those in group B (P < 0.05 or P < 0.01).
ECLP is better than HTK solution to preserve NHBD livers. ECLP can assess the graft viability before liver transplantation.
比较冷组氨酸 - 色氨酸 - 酮戊二酸(HTK)溶液和体外肝脏灌注(ECLP)对非心脏跳动供体(NHBD)肝脏的保存效果。
从健康猪获取的肝脏在冷HTK溶液中保存10小时(A组,n = 4)或在体温下用氧合自体血灌注(B组,n = 4)。然后两组均在体外循环装置上测试4小时。检测每组体外肝脏的胆汁生成、血流动力学参数、肝细胞标志物和再灌注损伤。再灌注结束时检查每组的肝组织。
再灌注后1、2、3和4小时,A组肝脏的胆汁生成、血流动力学参数、肝细胞标志物和再灌注损伤与B组相比有统计学差异(P < 0.05或P < 0.01)。
ECLP在保存NHBD肝脏方面优于HTK溶液。ECLP可在肝移植前评估移植物的活力。