Luisier Jean-Luc, Buettner Hermann, Völker Sebastian, Rausis Thierry, Frey Urban
Institute of Life Technologies, University of Applied Sciences Western Switzerland Valais, Route de Rawyl 64, CH-1950 Sion 2, Switzerland.
J Agric Food Chem. 2008 May 14;56(9):2883-7. doi: 10.1021/jf072963o. Epub 2008 Apr 17.
Making use of a convenient synthetic approach to prepare the deuterated S-3-(hexan-1-ol)-cysteine by a Michael addition reaction, an analytical method was developed to measure the presence of the cysteine S-conjugate, precursor of 3-sulfanylhexan-1-ol (3-mercaptohexan-1-ol), in must and wine from Petite Arvine vine. The method uses a stable isotope dilution assay with a suitable one-step sample preparation and HPLC-MS detection. The method has limits of detection and quantification of 3 and 10 microg/L, respectively. A correlation between the increase of the precursor concentration and the increase of the degree of rot has been established.
利用一种简便的合成方法，通过迈克尔加成反应制备氘代S-3-(己醇)-半胱氨酸，开发了一种分析方法，用于测定小阿维纳葡萄汁和葡萄酒中3-巯基己醇（3-硫代己醇）的前体——半胱氨酸S-共轭物的存在情况。该方法采用稳定同位素稀释分析法，具有合适的一步样品制备和高效液相色谱-质谱检测。该方法的检测限和定量限分别为3和10μg/L。已建立前体浓度增加与腐烂程度增加之间的相关性。
