Jayaraman T, Kannappan S, Ravichandran M K, Anuradha C V
Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Tamil Nadu 608002, India.
Singapore Med J. 2008 Apr;49(4):320-7.
The present study was designed to investigate the effect of Essentiale L, a mixture of polyenylphospholipids from soybeans, on oxidative stress in various brain regions, on erythrocytes (RBC) and on RBC membrane composition in ethanol-administered rats.
Adult male albino rats of body weight 150-170 g were divided into four groups and administered either isocaloric glucose (5 g/kg body weight/day) or ethanol (6 g/kg body weight/day) through oral gavage. Essentiale L was administered to a set of ethanol-fed rats and the control rats at a dosage of 300 mg/kg body weight/day through oral gavage. The treatment protocol was carried out for 45 days. At the end of the experimental period, the animals were sacrificed, and the biochemical parameters related to the lipid profile, oxidative stress and thiol status were assayed in the brain regions, RBC and RBC membrane.
Ethanol administration resulted in increased levels of lipid peroxidation products in RBC and different brain regions, such as the cortex, cerebellum, striatum, hippocampus and hypothalamus, and depletion of enzymatic and nonenzymatic antioxidants and alterations in oxidised glutathione/glutathione (GSSG/GSH) ratio and thiol groups (protein-bound and total), signifying oxidative stress. Ethanol-treated rats also showed significant alterations in protein content and lipid composition in RBC membranes. Significant differences in the relative proportions of hexose, hexosamine and sialic acid of the membranes were observed. Administration of Essentiale L prevented all the alterations induced by ethanol and returned their levels to near-normal.
These findings suggest that Essentiale L, a therapeutic adjunct for liver diseases, also has bioprotective effects on nonhepatic tissues and cells.
本研究旨在探讨易善力L（一种来自大豆的多烯磷脂混合物）对乙醇处理大鼠不同脑区、红细胞（RBC）及红细胞膜成分氧化应激的影响。
将体重150 - 170 g的成年雄性白化大鼠分为四组，通过灌胃给予等热量的葡萄糖（5 g/kg体重/天）或乙醇（6 g/kg体重/天）。对一组乙醇喂养的大鼠和对照大鼠通过灌胃给予易善力L，剂量为300 mg/kg体重/天。治疗方案持续45天。实验期结束时，处死动物，测定脑区、红细胞及红细胞膜中与血脂谱、氧化应激和硫醇状态相关的生化参数。
给予乙醇导致红细胞及不同脑区（如皮质、小脑、纹状体、海马体和下丘脑）脂质过氧化产物水平升高，酶促和非酶促抗氧化剂耗竭，氧化型谷胱甘肽/谷胱甘肽（GSSG/GSH）比值及硫醇基团（蛋白结合型和总硫醇）发生改变，表明存在氧化应激。乙醇处理的大鼠红细胞膜中的蛋白质含量和脂质组成也出现显著变化。观察到膜中己糖、己糖胺和唾液酸相对比例存在显著差异。给予易善力L可预防乙醇诱导的所有改变，并使其水平恢复至接近正常。
这些发现表明，作为肝病治疗辅助药物的易善力L对非肝组织和细胞也具有生物保护作用。
