印度对虾白斑综合征病毒可变基因组区域的特征分析

Characterization of variable genomic regions of Indian white spot syndrome virus.

作者信息

Pradeep Balakrishnan, Shekar Malathi, Karunasagar Iddya, Karunasagar Indrani

机构信息

Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore-575 002, India.

出版信息

Virology. 2008 Jun 20;376(1):24-30. doi: 10.1016/j.virol.2008.02.037.

DOI:10.1016/j.virol.2008.02.037

PMID:18420247

Abstract

A recent study examining genetic variations among the 3 completely sequenced white spot syndrome virus (WSSV) genomes isolated from China, Thailand and Taiwan revealed five major differences among them. Of these differences, a deletion region between ORF 23/24 and a variable region of ORF14/15 prone to recombination were of particular evolutionary significance. Focusing on these regions, 81 WSSV isolates from India were characterized by sequencing polymerase chain reaction (PCR) amplicons. The Indian strains carried a 10,970 bp deletion in the ORF 23/24 region relative to WSSV-TW and WSSV-TH-96-II. Analysis of the ORF 14/15 regions revealed two novel strains of WSSV with unique sequences which could have evolved by recombination. None of the WSSV isolates had a transposase sequence or VP35 gene as reported for Taiwan isolates. The Indian strains were closely related to Thailand strains suggesting movement of a putative ancestor from Thailand to other parts of the world including India.

摘要

最近一项对从中国、泰国和台湾分离出的3个完全测序的白斑综合征病毒（WSSV）基因组的遗传变异研究显示，它们之间存在五个主要差异。在这些差异中，ORF 23/24之间的一个缺失区域和易于重组的ORF14/15可变区域具有特别的进化意义。针对这些区域，通过对聚合酶链反应（PCR）扩增子进行测序，对来自印度的81株WSSV分离株进行了特征分析。相对于WSSV-TW和WSSV-TH-96-II，印度菌株在ORF 23/24区域存在一个10970 bp的缺失。对ORF 14/15区域的分析揭示了两株具有独特序列的新型WSSV菌株，它们可能是通过重组进化而来。没有一株WSSV分离株具有台湾分离株所报道的转座酶序列或VP35基因。印度菌株与泰国菌株密切相关，这表明一个假定的祖先从泰国传播到了包括印度在内的世界其他地区。