Wen Yan, Zhao Yang-Yu, Polan Mary Lake, Chen Bertha
Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California 94305, USA.
Reprod Sci. 2008 Apr;15(3):312-20. doi: 10.1177/1933719108315299.
The objective of this study is to compare relaxin's effect on transforming growth factor (TGF)- beta1 and latent TGF-beta1-binding protein (LTBP-1) in vaginal fibroblasts from women with stress urinary incontinence (SUI) to continent women (controls) in both phases of the menstrual cycle. Fibroblasts were treated with relaxin. TGF-beta1 levels were measured by enzyme-linked immunosorbent assay. LTBP-1 expression was evaluated by Western blot. In the proliferative phase, total TGF-beta1 level in the supernatant, cells, and extracellular matrix (ECM) of SUI fibroblasts decreased with increasing relaxin concentration (P < .05). Active TGF-beta1 levels increased at a low concentration of relaxin (P < .05) in the supernatant but decreased in the ECM of SUI fibroblasts at high concentration (P < .05). In the secretory phase, total TGF-beta1 levels decreased with relaxin treatment (P < .05) in the supernatant, cells and ECM of both women with SUI and controls. Relaxin decreased the levels of total and active TGF-beta1 in the ECM isolated from SUI vaginal fibroblasts.
本研究的目的是比较在月经周期的两个阶段，松弛素对压力性尿失禁（SUI）女性与尿失禁正常女性（对照组）阴道成纤维细胞中转化生长因子（TGF）-β1和潜伏性TGF-β1结合蛋白（LTBP-1）的影响。用松弛素处理成纤维细胞。通过酶联免疫吸附测定法测量TGF-β1水平。通过蛋白质印迹法评估LTBP-1表达。在增殖期，SUI成纤维细胞的上清液、细胞和细胞外基质（ECM）中的总TGF-β1水平随着松弛素浓度的增加而降低（P <.05）。在低浓度松弛素作用下，上清液中活性TGF-β1水平升高（P <.05），但在高浓度时SUI成纤维细胞的ECM中活性TGF-β1水平降低（P <.05）。在分泌期，SUI女性和对照组女性的上清液、细胞和ECM中，经松弛素处理后总TGF-β1水平均降低（P <.05）。松弛素降低了从SUI阴道成纤维细胞分离的ECM中总TGF-β1和活性TGF-β1的水平。
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