在委内瑞拉链霉菌中异源表达metK1-sp和afsR-sp以生产匹克霉素。
Heterologous expression of metK1-sp and afsR-sp in Streptomyces venezuelae for the production of pikromycin.
作者信息
Maharjan Sushila, Oh Tae-Jin, Lee Hei Chan, Sohng Jae Kyung
机构信息
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam 336-708, Republic of Korea.
出版信息
Biotechnol Lett. 2008 Sep;30(9):1621-6. doi: 10.1007/s10529-008-9735-0. Epub 2008 Apr 18.
Two regulator genes, metK1-sp and afsR-sp, from Streptomyces peucetius ATCC 27952 were heterologously expressed in S. venezuelae ATCC 15439, to produce 14-membered pikromycin antibiotics. The production of pikromycin was increased by 1.6-fold and 2.6-fold by the expression of metK1-sp and afsR-sp, respectively. The overexpression of metK1-sp and afsR-sp in S. venezuelae stimulated the expression of the pathway-specific regulatory genes, pikD and ketosynthase, as demonstrated by RT-PCR. The elevated transcripts of the pikD and ketosynthase genes were consistent with the enhanced production of pikromycin.
来自变铅青链霉菌ATCC 27952的两个调控基因metK1-sp和afsR-sp在委内瑞拉链霉菌ATCC 15439中进行了异源表达，以产生14元的匹克霉素类抗生素。分别通过metK1-sp和afsR-sp的表达，匹克霉素的产量提高了1.6倍和2.6倍。如RT-PCR所示，metK1-sp和afsR-sp在委内瑞拉链霉菌中的过表达刺激了途径特异性调控基因pikD和酮合成酶的表达。pikD和酮合成酶基因转录本的增加与匹克霉素产量的提高相一致。
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