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来自北极分离株的联苯双加氧酶并非冷适应型。

Biphenyl dioxygenase from an arctic isolate is not cold adapted.

作者信息

Master Emma R, Agar Nathalie Y R, Gómez-Gil Leticia, Powlowski Justin B, Mohn William W, Eltis Lindsay D

机构信息

Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.

出版信息

Appl Environ Microbiol. 2008 Jun;74(12):3908-11. doi: 10.1128/AEM.02928-07. Epub 2008 Apr 18.

DOI:10.1128/AEM.02928-07
PMID:18424535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2446576/
Abstract

Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO(Cam-1)) was purified and found to have an apparent k(cat) for biphenyl of 1.1 +/- 0.1 s(-1) (mean +/- standard deviation) at 4 degrees C. In contrast, BPDO(LB400) from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57 degrees C, the half-life of the BPDO(Cam-1) oxygenase was less than half that of the BPDO(LB400) oxygenase. Nevertheless, BPDO(Cam-1) appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase.

摘要

从耐冷细菌假单胞菌属菌株Cam-1中纯化得到的联苯双加氧酶（BPDO(Cam-1)），发现在4℃时对联苯的表观催化常数k(cat)为1.1±0.1 s(-1)（平均值±标准差）。相比之下，嗜温菌洋葱伯克霍尔德菌LB400的BPDO(LB400)在该温度下未检测到活性。在57℃时，BPDO(Cam-1)加氧酶的半衰期不到BPDO(LB400)加氧酶半衰期的一半。然而，BPDO(Cam-1)似乎是典型的类产碱假单胞菌KF707型双加氧酶。

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