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鲤鱼(Cyprinus carpio)中不同的细胞色素P450芳香化酶亚型:性别二态性及个体发育表达的起始

Distinct cytochrome P450 aromatase isoforms in the common carp (Cyprinus carpio): sexual dimorphism and onset of ontogenic expression.

作者信息

Barney Megan L, Patil Jawahar G, Gunasekera Rasanthi M, Carter Chris G

机构信息

CSIRO, Marine and Atmospheric Research, Castray Esplanade, Hobart, Tasmania 7000, Australia.

出版信息

Gen Comp Endocrinol. 2008 May 1;156(3):499-508. doi: 10.1016/j.ygcen.2008.03.013. Epub 2008 Mar 25.

Abstract

Cytochrome P450 aromatase (CYP19) is a key enzyme in the steroidogenic pathway that catalyses the conversion of testosterone to estrogen, and therefore is thought to influence gonadal sex differentiation. In an effort to understand the role of this enzyme in ovarian differentiation, we isolated cDNA encoding the two distinct isoforms, ovarian and brain (termed cyp19a and cyp19b, respectively) of adult common carp, Cyprinus carpio. The cloned cDNA for cyp19a had an open reading frame (ORF) of 518 amino acid residues, in contrast to cyp19b with an ORF of 511 amino acids. Sequence and phylogenetic analysis showed that these CYP19 isoforms were orthologous with previously described cyp19a and cyp19b from other teleosts. Quantitative real-time PCR indicated that both isoforms are expressed in adult ovary and brain, with predominant expression of cyp19a in the ovary and cyp19b in the brain. The major aromatase expressing tissue was found to be the brain, with greatest cyp19b expression in the anterior quarter (telencephalon) in both sexes. The gonad showed sexually dimorphic expression of both genes and dimorphic expression of cyp19a was observed in the cerebellum and the liver. Ontogenic expression showed that only the ovarian aromatase transcript is inherited maternally, with lower expression observed through early larval development under warmer rearing conditions. The differential and overlapping expression suggests these two aromatase genes have different roles in reproductive physiology.

摘要

细胞色素P450芳香化酶(CYP19)是类固醇生成途径中的一种关键酶,它催化睾酮向雌激素的转化,因此被认为会影响性腺性别分化。为了了解这种酶在卵巢分化中的作用,我们分离了成年鲤鱼(Cyprinus carpio)中编码两种不同同工型(分别称为cyp19a和cyp19b)的cDNA,这两种同工型分别存在于卵巢和大脑中。cyp19a的克隆cDNA具有一个由518个氨基酸残基组成的开放阅读框(ORF),而cyp19b的ORF由511个氨基酸组成。序列和系统发育分析表明,这些CYP19同工型与先前描述的其他硬骨鱼的cyp19a和cyp19b是直系同源的。定量实时PCR表明,这两种同工型在成年卵巢和大脑中均有表达,cyp19a在卵巢中占主导表达,cyp19b在大脑中占主导表达。发现主要表达芳香化酶的组织是大脑,在两性的前四分之一(端脑)中cyp19b表达最高。性腺显示出这两个基因的性二态性表达,并且在小脑和肝脏中观察到cyp19a的二态性表达。个体发育表达表明,只有卵巢芳香化酶转录本是母系遗传的,在温暖饲养条件下的早期幼体发育过程中表达较低。差异和重叠表达表明这两个芳香化酶基因在生殖生理学中具有不同的作用。

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