用于分离十二烷基硫酸钠 - 蛋白质复合物的聚甲基丙烯酸甲酯微芯片的冲洗与蒸发涂层

Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate-protein complex.

作者信息

Okada Hiroki, Kaji Noritada, Tokeshi Manabu, Baba Yoshinobu

机构信息

Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

出版信息

J Chromatogr A. 2008 May 30;1192(2):289-93. doi: 10.1016/j.chroma.2008.03.012. Epub 2008 Mar 18.

DOI:10.1016/j.chroma.2008.03.012

PMID:18430430

Abstract

We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 x 10(-5)cm(2)V(-1)s(-1)) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.

摘要

我们使用甲基纤维素（MC）作为涂层试剂，在聚甲基丙烯酸甲酯（PMMA）微芯片上开发了一种新型的通道壁涂层，以抑制电渗流（EOF）。这是通过MC与PMMA通道壁之间的疏水相互作用，使强分析物吸附之后实现的。我们的涂层是通过先用含MC的水溶液冲洗通道壁，然后蒸发而获得的。该涂层使亲水性通道壁的EOF降低了两个数量级（1.2×10⁻⁵cm²V⁻¹s⁻¹），同时减少了疏水吸附。在涂覆的通道壁上，我们使用葡聚糖作为低粘度蛋白质分离介质，成功地以高重现性和效率分离了十二烷基硫酸钠-蛋白质复合物。