利用响应面法优化枯草芽孢杆菌产纳豆激酶的培养基成分
Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology.
作者信息
Deepak V, Kalishwaralal K, Ramkumarpandian S, Babu S Venkatesh, Senthilkumar S R, Sangiliyandi G
机构信息
Department of Biotechnology, Kalasalingam University, Krishnankoil 626190, Viruthunagar District, Tamil Nadu, India.
出版信息
Bioresour Technol. 2008 Nov;99(17):8170-4. doi: 10.1016/j.biortech.2008.03.018. Epub 2008 Apr 21.
Response surface methodology and central composite rotary design (CCRD) was employed to optimize a fermentation medium for the production of Nattokinase by Bacillus subtilis at pH 7.5. The four variables involved in this study were Glucose, Peptone, CaCl2, and MgSO4. The statistical analysis of the results showed that, in the range studied; only peptone had a significant effect on Nattokinase production. The optimized medium containing (%) Glucose: 1, Peptone: 5.5, MgSO4: 0.2 and CaCl2: 0.5 resulted in 2-fold increased level of Nattokinase (3194.25U/ml) production compared to initial level (1599.09U/ml) after 10h of fermentation. Nattokinase production was checked with fibrinolytic activity.
采用响应面法和中心复合旋转设计（CCRD）对枯草芽孢杆菌在pH 7.5条件下生产纳豆激酶的发酵培养基进行优化。本研究涉及的四个变量为葡萄糖、蛋白胨、氯化钙和硫酸镁。结果的统计分析表明，在所研究的范围内，只有蛋白胨对纳豆激酶的产生有显著影响。优化后的培养基含有（%）葡萄糖：1、蛋白胨：5.5、硫酸镁：0.2和氯化钙：0.5，与发酵10小时后的初始水平（1599.09U/ml）相比，纳豆激酶产量提高了2倍（3194.25U/ml）。通过纤维蛋白溶解活性检测纳豆激酶的产生。
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