人和小鼠白细胞介素-12的测量
Measurement of human and mouse interleukin-12.
作者信息
Gately Maurice K, Chizzonite Richard, Presky David H
机构信息
Hoffmann-La Roche Inc, Nutley, New Jersey.
出版信息
Curr Protoc Immunol. 2001 May;Chapter 6:6.16.1-6.16.15. doi: 10.1002/0471142735.im0616s15.
PMID:18432807
Abstract
This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts ("PHA blasts"). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.
摘要
本单元描述了用于测量生物活性白细胞介素-12（IL-12）的功能测定法以及用于测量IL-12蛋白的酶联免疫吸附测定法（ELISA）。功能测定法基于IL-12刺激PHA激活的T淋巴母细胞（“PHA母细胞”）增殖的能力。ELISA在技术上比功能测定法更易于操作，但无法区分具有生物活性和无活性的细胞因子。
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