Cheng Cheng, Wei Hua, Zhang Xian-Zheng, Cheng Si-Xue, Zhuo Ren-Xi
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
J Biomed Mater Res A. 2009 Mar 1;88(3):814-22. doi: 10.1002/jbm.a.31770.
Thermosensitive and biotinylated biotin-poly (N-isopropylacrylamide-co-N-hydroxymethylacrylamide)-block-poly(methyl methacrylate) (biotin-P(NIPAAm-co-HMAAm)-b-PMMA) block copolymers were designed and synthesized. The conjugation of biotin molecule with the copolymer as well as the capability of easily functionalizing with ligands for pretargeting approach of the biotinylated multifunctional drug carrier was confirmed by a novel method called capillary electrophoresis immunoassay (CEIA) based on enhanced chemiluminescence (CL) detection. The biotin-P(NIPAAm-co-HMAAm)-b-PMMA copolymer was capable of self-assembling into nanometer-sized micelle. The anticancer drug methotrexate (MTX), used as a model drug, was loaded in the self-assembled micelles and the thermo-triggered release behavior of MTX was investigated.
设计并合成了热敏且生物素化的生物素-聚（N-异丙基丙烯酰胺-co-N-羟甲基丙烯酰胺）-嵌段-聚（甲基丙烯酸甲酯）（生物素-P（NIPAAm-co-HMAAm）-b-PMMA）嵌段共聚物。通过一种基于增强化学发光（CL）检测的新型方法——毛细管电泳免疫分析（CEIA），证实了生物素分子与共聚物的缀合以及生物素化多功能药物载体预靶向方法中与配体轻松功能化的能力。生物素-P（NIPAAm-co-HMAAm）-b-PMMA共聚物能够自组装成纳米尺寸的胶束。将抗癌药物甲氨蝶呤（MTX）用作模型药物，负载于自组装胶束中，并研究了MTX的热触发释放行为。
