Rex1/Zfp42对小鼠胚胎干细胞的多能性并非不可或缺。
Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.
作者信息
Masui Shinji, Ohtsuka Satoshi, Yagi Rika, Takahashi Kadue, Ko Minoru S H, Niwa Hitoshi
机构信息
Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology (CDB), 2-2-3 Minatojima-minamimachi, Kobe, Hyogo 650-0047, Japan.
出版信息
BMC Dev Biol. 2008 Apr 24;8:45. doi: 10.1186/1471-213X-8-45.
BACKGROUND
Rex1/Zfp42 has been extensively used as a marker for the undifferentiated state of pluripotent stem cells. However, its function in pluripotent stem cells including embryonic stem (ES) cells remained unclear although its involvement in visceral endoderm differentiation in F9 embryonal carcinoma (EC) cells was reported.
RESULTS
We showed the function of Rex1 in mouse ES cells as well as in embryos using the conventional gene targeting strategy. Our results clearly indicated that Rex1 function is dispensable for both the maintenance of pluripotency in ES cells and the development of embryos. However, Rex1-/- ES cells showed the defect to induce a subset of the marker genes of visceral endoderm, when differentiated as embryoid body, as found in EC cells.
CONCLUSION
Rex1 should be regarded just as a marker of pluripotency without functional significance like the activity of alkaline phosphatase.
背景
Rex1/Zfp42已被广泛用作多能干细胞未分化状态的标志物。然而，尽管有报道称其参与F9胚胎癌细胞的内胚层分化，但其在包括胚胎干细胞（ES细胞）在内的多能干细胞中的功能仍不清楚。
结果
我们使用传统的基因靶向策略展示了Rex1在小鼠ES细胞以及胚胎中的功能。我们的结果清楚地表明，Rex1的功能对于ES细胞多能性的维持和胚胎发育都是可有可无的。然而，如在胚胎癌细胞中所发现的那样，当分化为胚状体时，Rex1基因敲除的ES细胞显示出诱导内胚层部分标志物基因的缺陷。
结论
Rex1应被视为仅仅是多能性的一个标志物，而没有像碱性磷酸酶活性那样的功能意义。
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