使用玫瑰花环抑制试验检测奶牛妊娠时的早期妊娠因子（EPF）测定

Measurement of EPF for detection of cow pregnancy using rosette inhibition test.

作者信息

Ghaffari Laleh V, Ghaffari Laleh R, Pirany N, Moghadaszadeh Ahrabi M

机构信息

Young Researchers Club of Tabriz, Islamic Azad University of Tabriz, Tabriz, East Azerbaijan, Iran.

出版信息

Theriogenology. 2008 Jul 1;70(1):105-7. doi: 10.1016/j.theriogenology.2008.02.003. Epub 2008 Apr 22.

DOI:10.1016/j.theriogenology.2008.02.003

PMID:18433850

Abstract

Early embryonic death of calves due to sub-fertility in cows is of great economic concern to dairy industry. Early pregnancy factor (EPF) is a secretory protein with pregnancy associated immunosuppressive properties. Rosette inhibition test (RIT) was used to detect EPF in inseminated dairy cows. Blood samples were collected at two intervals, 1-3 and 5-7 days after insemination from 23 inseminated and 18 non-inseminated control cows for RIT and pregnancy diagnosis performed between 42 and 45 days on palpation. The study indicates that RIT (P<0.05) has the potential to distinguish pregnant from non-pregnant dairy cows in the first week of pregnancy.

摘要

奶牛亚生育导致的犊牛早期胚胎死亡对乳制品行业而言是重大经济问题。早期妊娠因子（EPF）是一种具有妊娠相关免疫抑制特性的分泌蛋白。采用玫瑰花环抑制试验（RIT）检测人工授精奶牛体内的EPF。在人工授精后1 - 3天和5 - 7天这两个时间点，从23头人工授精奶牛和18头未授精的对照奶牛采集血样进行RIT检测，并在42至45天进行触诊妊娠诊断。该研究表明，RIT（P<0.05）有潜力在妊娠第一周区分妊娠奶牛和未妊娠奶牛。