Chua Anna C N, Jiang Pei-Luen, Shi Li-Shian, Chou Wing-Ming, Tzen Jason T C
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, Taiwan.
Plant Physiol Biochem. 2008 May-Jun;46(5-6):525-32. doi: 10.1016/j.plaphy.2008.03.001. Epub 2008 Mar 18.
Thin-layer chromatography analysis revealed that the contents stored in oil bodies isolated from jelly fig (Ficus awkeotsang Makino) achenes were mainly neutral lipids (>90% triacylglycerols and approximately 5% diacylglycerols). Fatty acids released from the neutral lipids of achene oil bodies were highly unsaturated (62.65% alpha-linolenic acid, 18.24% linoleic acid, and 10.62% oleic acid). The integrity of isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms and one caleosin were present in these oil bodies. MALDI-MS analyses confirmed that the three full-length cDNA fragments obtained by PCR cloning from maturing achenes encoded the two jelly fig oleosin isoforms and one caleosin identified by immunological screening.
薄层层析分析表明，从爱玉（Ficus awkeotsang Makino）瘦果中分离出的油体中储存的物质主要是中性脂质（>90%为三酰甘油，约5%为二酰甘油）。瘦果油体中性脂质释放的脂肪酸高度不饱和（62.65%为α-亚麻酸，18.24%为亚油酸，10.62%为油酸）。分离出的油体的完整性可能是通过其表面蛋白提供的负电排斥和空间位阻来维持的。使用抗芝麻油体蛋白的抗体进行的免疫交叉识别表明，这些油体中存在两种油质蛋白异构体和一种钙结合蛋白。基质辅助激光解吸电离质谱分析证实，通过PCR克隆从成熟瘦果中获得的三个全长cDNA片段编码了通过免疫筛选鉴定出的两种爱玉油质蛋白异构体和一种钙结合蛋白。
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