Baerenfaller Katja, Grossmann Jonas, Grobei Monica A, Hull Roger, Hirsch-Hoffmann Matthias, Yalovsky Shaul, Zimmermann Philip, Grossniklaus Ueli, Gruissem Wilhelm, Baginsky Sacha
Institute of Plant Sciences, ETH (Swiss Federal Institute of Technology) Zurich, Universitätstrasse 2, 8092 Zurich, Switzerland.
Science. 2008 May 16;320(5878):938-41. doi: 10.1126/science.1157956. Epub 2008 Apr 24.
We have assembled a proteome map for Arabidopsis thaliana from high-density, organ-specific proteome catalogs that we generated for different organs, developmental stages, and undifferentiated cultured cells. We matched 86,456 unique peptides to 13,029 proteins and provide expression evidence for 57 gene models that are not represented in the TAIR7 protein database. Analysis of the proteome identified organ-specific biomarkers and allowed us to compile an organ-specific set of proteotypic peptides for 4105 proteins to facilitate targeted quantitative proteomics surveys. Quantitative information for the identified proteins was used to establish correlations between transcript and protein accumulation in different plant organs. The Arabidopsis proteome map provides information about genome activity and proteome assembly and is available as a resource for plant systems biology.
我们从为不同器官、发育阶段和未分化培养细胞生成的高密度、器官特异性蛋白质组目录中，组装了一份拟南芥蛋白质组图谱。我们将86456个独特肽段与13029个蛋白质进行了匹配，并为TAIR7蛋白质数据库中未出现的57个基因模型提供了表达证据。蛋白质组分析鉴定出了器官特异性生物标志物，并使我们能够为4105个蛋白质编制一组器官特异性的蛋白质型肽段，以促进靶向定量蛋白质组学研究。所鉴定蛋白质的定量信息用于建立不同植物器官中转录本和蛋白质积累之间的相关性。拟南芥蛋白质组图谱提供了有关基因组活性和蛋白质组组装的信息，可作为植物系统生物学的资源使用。
