Gabel Harrison W, Ruvkun Gary
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, CPZN7250, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
Nat Struct Mol Biol. 2008 May;15(5):531-3. doi: 10.1038/nsmb.1411. Epub 2008 Apr 27.
The exonuclease ERI-1 negatively regulates RNA interference in Caenorhabditis elegans and Schizosaccharomyces pombe, and is required for production of some C. elegans endogenous small interfering RNAs. We show that ERI-1 performs 3' end processing of the 5.8S ribosomal RNA in both C. elegans and S. pombe. In C. elegans, two protein isoforms of ERI-1 are localized to the cytoplasm, and each has distinct functions in ribosomal RNA processing and negative regulation of RNA interference.
核酸外切酶ERI-1对秀丽隐杆线虫和粟酒裂殖酵母中的RNA干扰起负调控作用,并且是某些秀丽隐杆线虫内源性小干扰RNA产生所必需的。我们发现,ERI-1在秀丽隐杆线虫和粟酒裂殖酵母中都参与5.8S核糖体RNA的3'末端加工。在秀丽隐杆线虫中,ERI-1的两种蛋白质异构体定位于细胞质,并且它们在核糖体RNA加工和RNA干扰的负调控中各自具有不同的功能。