Schopf Simone, Wanner Gerhard, Rachel Reinhard, Wirth Reinhard
Mikrobiologie-NWF III, Archaeenzentrum, Universität Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
Arch Microbiol. 2008 Sep;190(3):371-7. doi: 10.1007/s00203-008-0371-9. Epub 2008 Apr 26.
Recently it was shown that Pyrococcus furiosus uses its flagella not only for swimming, but also for establishment of cell-cell connections, and for adhesion to abiotic surfaces. Therefore, it was asked here if P. furiosus might be able to adhere also to biotic surfaces. Since Methanopyrus kandleri can be found in habitats similar to those of P. furiosus (seawater close to the boiling point and anaerobic conditions) it was tested if interactions between both archaea occur. Using a standard medium and a gas phase reduced in H2 (compared with the optimal gas phase for M. kandleri) we were able to grow both species in a stable coculture. Very interestingly, M. kandleri could adhere to glass under such conditions, but not P. furiosus. This latter archaeum, however, was able to adhere onto M. kandleri cells and onto itself, resulting in structured biofilms on glass. These very often appeared as a bottom layer of M. kandleri cells covered by a multitude of P. furiosus cells. Interactions between P. furiosus and M. kandleri were mediated not only by flagella, but also by direct cell-cell contact.
最近有研究表明，激烈火球菌（Pyrococcus furiosus）的鞭毛不仅用于游动，还用于建立细胞间连接以及附着在非生物表面。因此，本文探讨了激烈火球菌是否也能附着在生物表面。由于坎氏甲烷球菌（Methanopyrus kandleri）存在于与激烈火球菌相似的生境中（接近沸点的海水和厌氧条件），因此测试了这两种古菌之间是否会发生相互作用。使用标准培养基和氢气含量降低的气相（与坎氏甲烷球菌的最佳气相相比），我们能够使这两种菌在稳定的共培养体系中生长。非常有趣的是，在这种条件下坎氏甲烷球菌能够附着在玻璃上，而激烈火球菌则不能。然而，后一种古菌能够附着在坎氏甲烷球菌细胞上以及自身细胞上，从而在玻璃上形成结构化生物膜。这些生物膜通常表现为一层坎氏甲烷球菌细胞，上面覆盖着大量的激烈火球菌细胞。激烈火球菌与坎氏甲烷球菌之间的相互作用不仅由鞭毛介导，还通过细胞间直接接触介导。
