IS1096介导的DNA重排在耻垢分枝杆菌的基因组进化中起关键作用。
IS1096-mediated DNA rearrangements play a key role in genome evolution of Mycobacterium smegmatis.
作者信息
Wang Xiao-Ming, Galamba Alexandra, Warner Digby F, Soetaert Karine, Merkel Jane S, Kalai Michael, Bifani Pablo, Lefèvre Philippe, Mizrahi Valerie, Content Jean
机构信息
IPH-Pasteur Institute of Brussels, Rue Engeland 642, Brussels, Belgium.
出版信息
Tuberculosis (Edinb). 2008 Sep;88(5):399-409. doi: 10.1016/j.tube.2008.02.003. Epub 2008 Apr 24.
The acquisition of DNA and the loss of genetic information are two important mechanisms that contribute to strain-specific differences in genome content. In this study, comparative genomics has allowed us to infer the roles of genomic rearrangement and changes in both distribution and copy number of the insertion element, IS1096, in the evolution of Mycobacterium smegmatis mc2155 from its progenitor, M. smegmatis ATCC 607. Comparative analysis revealed that the ATCC 607 genome contains only 11 IS1096 elements against the 24 reported in mc2155. As mc2155 evolved, there was a considerable expansion in the copy number of IS1096 (+13) as well as duplication of a 56-kb fragment flanked on both sides by IS1096; concurrently, a single IS1096 element and its flank were deleted. This study demonstrates that insertion sequence (IS) expansion and IS-induced rearrangements such as duplication, deletion and shuffling are major forces driving genomic diversity and evolution.
DNA的获取和遗传信息的丢失是导致基因组内容存在菌株特异性差异的两个重要机制。在本研究中，比较基因组学使我们能够推断基因组重排以及插入元件IS1096的分布和拷贝数变化在耻垢分枝杆菌mc2155从其祖先耻垢分枝杆菌ATCC 607进化过程中的作用。比较分析表明，ATCC 607基因组仅含有11个IS1096元件，而mc2155中报道有24个。随着mc2155的进化，IS1096的拷贝数显著增加（增加了13个），同时两侧由IS1096侧翼的一个56 kb片段发生了重复；与此同时，一个单一的IS1096元件及其侧翼被删除。本研究表明，插入序列（IS）的扩增以及IS诱导的重排，如重复、缺失和改组，是推动基因组多样性和进化的主要力量。
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