Chruszcz Maksymilian, Wlodawer Alexander, Minor Wladek
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.
Biophys J. 2008 Jul;95(1):1-9. doi: 10.1529/biophysj.108.131789. Epub 2008 Apr 25.
Determination of a macromolecular structure using x-ray diffraction is a multistep process that involves a plethora of techniques involving molecular biology, bioinformatics, and physical sciences. Counterintuitively, the success of any or all individual steps does not guarantee the success of the overall process. This review examines the difficulties presented by each step on the path from a gene to the final publication, together with certain lucky (or unlucky) circumstances that can affect the velocity along that path.
使用X射线衍射确定大分子结构是一个多步骤过程,涉及大量分子生物学、生物信息学和物理科学方面的技术。与直觉相反的是,任何一个或所有单个步骤的成功并不能保证整个过程的成功。本综述探讨了从基因到最终发表过程中每个步骤所面临的困难,以及可能影响该过程进展速度的某些幸运(或不幸)情况。