丝裂原活化蛋白激酶和核因子-κB激活在一氧化氮诱导人牙髓细胞白细胞介素-8表达中的作用

Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide-induced interleukin-8 expression in human pulp cells.

作者信息

Min Kyung-San, Kim Hyun-Il, Chang Hoon-Sang, Kim Hyung-Ryong, Pae Hyun-Ock, Chung Hun-Taeg, Hong Seung-Heon, Shin Hong-In, Hong Chan-Ui, Lee Suk-Keun, Kim Eun-Cheol

机构信息

Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Iksan, South Korea.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 May;105(5):654-60. doi: 10.1016/j.tripleo.2007.11.011.

DOI:10.1016/j.tripleo.2007.11.011

PMID:18442745

Abstract

OBJECTIVE

This study examined the effect of nitric oxide (NO) on interleukin-8 (IL-8) production and the involvement of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappaB) signaling pathways in primary cultured human pulp cells.

STUDY DESIGN

IL-8 production was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAPK activation and IkappaB degradation and phosphorylation were determined by western blotting.

RESULTS

Sodium nitroprusside (SNP), an NO donor, has increased IL-8 secretion and mRNA expression in a dose- and time-dependent manner. SNP induced the phosphorylation of p38 MAPK and extracellular-regulated kinase (ERK), degradation and phosphorylation of IkappaB, and activation of NF-kappaB. Furthermore, inhibition of the ERK, p38, and NF-kappaB pathways blocked SNP-induced IL-8 secretion.

CONCLUSION

Human pulp cells showed NO-induced IL-8 expression via the MAPK and NF-kappaB pathways, which may play an important role in the inflammatory responses of pulp and periapical lesions.

摘要