小鼠中细胞周期蛋白D1b信使核糖核酸变体的鉴定。
Identification of the cyclin D1b mRNA variant in mouse.
作者信息
Wu Jack, Wu Si-hung, Bollig Aliccia, Thakur Archana, Liao D Joshua
机构信息
Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA.
出版信息
Mol Biol Rep. 2009 May;36(5):953-7. doi: 10.1007/s11033-008-9267-7. Epub 2008 Apr 30.
Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA.
细胞周期蛋白D1在细胞周期的G1期发挥关键的调控作用，其基因在许多癌症中发生扩增并过度表达。细胞周期蛋白D1b mRNA变体已在人类细胞中得到确认，最近的功能分析表明其蛋白质产物在人类癌细胞中具有独特的活性。通过进行逆转录-聚合酶链反应（RT-PCR）和cDNA末端快速扩增（RACE）实验，我们在小鼠中鉴定出了细胞周期蛋白D1b mRNA变体。与其人类对应物相似，小鼠细胞周期蛋白D1b转录本由外显子1、2、3、4和部分内含子4组成，并包含一个长开放阅读框（ORF）。该ORF预测的肽段比人类细胞周期蛋白D1b长34个氨基酸。我们对这种小鼠mRNA变体的表达进行了研究。它似乎在各种小鼠细胞系和组织中普遍且差异表达，其水平可能与经典的内源性细胞周期蛋白D1a mRNA的水平成比例。
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