Singh Sanjleena, Jones Ben J, Crawford Ross, Xiao Yin
Bone Tissue Engineering Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
Stem Cells Dev. 2008 Apr;17(2):245-54. doi: 10.1089/scd.2007.0146.
Osteophytes are a distinct feature of osteoarthritis (OA). Their formation may be related to pluripotential cells in the periosteum responding to stimulus during OA. This study aimed to isolate stem cells from osteophyte tissues and to characterize their phenotype, proliferation, and differentiation potential, as well as their immunomodulatory properties. Osteophyte-derived cells were isolated from osteophyte tissue samples collected during knee replacement surgery. These cells were characterized by the expression of cell-surface antigens, differentiation potential into mesenchymal lineages, growth kinetics, and modulation of alloimmune responses. Multipotential stem cells were identified from all osteophyte samples, namely osteophyte-derived mesenchymal stem cells (oMSCs). The surface antigen expression of oMSCs was consistent with that of MSCs; they lacked the hematopoietic and common leukocyte markers (CD34, CD45) while expressing those related to adhesion (CD29, CD166, CD44) and stem cells (CD90, CD105, CD73). The proliferation capacity of oMSCs in culture was superior to that of bone marrow-derived MSCs (bMSCs), and these cells readily differentiated into tissues of the mesenchymal lineages. oMSCs also demonstrated the ability to suppress allogeneic T cell proliferation, which was associated with the expression of the tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO). Our results showed that osteophyte-derived cells had similar properties to MSCs in the expression of antigen phenotype, differential potential, and suppression of alloimmune response. Furthermore, when compared to bMSCs, oMSCs maintained a higher proliferative capacity, which may offer new insights of the tissue formation and potentially an alternative source for therapeutic stem cell-based tissue regeneration.
骨赘是骨关节炎（OA）的一个显著特征。其形成可能与骨膜中的多能细胞在OA期间对刺激的反应有关。本研究旨在从骨赘组织中分离干细胞，并对其表型、增殖、分化潜能以及免疫调节特性进行表征。从膝关节置换手术中收集的骨赘组织样本中分离出骨赘来源的细胞。这些细胞通过细胞表面抗原的表达、向间充质谱系的分化潜能、生长动力学以及同种异体免疫反应的调节来表征。从所有骨赘样本中鉴定出多能干细胞，即骨赘来源的间充质干细胞（oMSCs）。oMSCs的表面抗原表达与MSCs一致；它们缺乏造血和常见白细胞标志物（CD34、CD45），同时表达与粘附（CD29、CD166、CD44）和干细胞（CD90、CD105、CD73）相关的标志物。oMSCs在培养中的增殖能力优于骨髓来源的MSCs（bMSCs），并且这些细胞易于分化为间充质谱系的组织。oMSCs还表现出抑制同种异体T细胞增殖的能力，这与色氨酸降解酶吲哚胺2,3-双加氧酶（IDO）的表达有关。我们的结果表明，骨赘来源的细胞在抗原表型表达、分化潜能和抑制同种异体免疫反应方面与MSCs具有相似的特性。此外，与bMSCs相比，oMSCs保持了更高的增殖能力，这可能为组织形成提供新的见解，并可能为基于治疗性干细胞的组织再生提供替代来源。
