Suppr超能文献

严重急性呼吸综合征冠状病毒的核衣壳蛋白通过与翻译延伸因子1α相互作用来抑制细胞胞质分裂和增殖。

The nucleocapsid protein of severe acute respiratory syndrome coronavirus inhibits cell cytokinesis and proliferation by interacting with translation elongation factor 1alpha.

作者信息

Zhou Bing, Liu Junli, Wang Qiuna, Liu Xuan, Li Xiaorong, Li Ping, Ma Qingjun, Cao Cheng

机构信息

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.

出版信息

J Virol. 2008 Jul;82(14):6962-71. doi: 10.1128/JVI.00133-08. Epub 2008 Apr 30.

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N) protein of SARS-CoV as a bait, the C terminus (amino acids 251 to 422) of the N protein was found to interact with human elongation factor 1-alpha (EF1alpha), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous actin (F-actin). In vitro and in vivo interaction was then confirmed by immuno-coprecipitation, far-Western blotting, and surface plasmon resonance. It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1alpha, inhibiting protein translation and cytokinesis by blocking F-actin bundling. Proliferation of human peripheral blood lymphocytes and other human cell lines was significantly inhibited by the infection of recombinant retrovirus expressing SARS-CoV N protein.

摘要

严重急性呼吸综合征冠状病毒(SARS-CoV)是SARS的病原体,SARS是一种以非典型肺炎为特征的新出现疾病。利用酵母双杂交筛选,以SARS-CoV的核衣壳(N)蛋白作为诱饵,发现N蛋白的C末端(氨基酸251至422)与人类延伸因子1-α(EF1α)相互作用,EF1α是翻译机制的一个重要组成部分,在胞质分裂中起重要作用,促进丝状肌动蛋白(F-肌动蛋白)的成束。然后通过免疫共沉淀、远缘Western印迹和表面等离子体共振证实了体外和体内的相互作用。结果表明,SARS-CoV的N蛋白诱导EF1α聚集,通过阻断F-肌动蛋白成束抑制蛋白质翻译和胞质分裂。表达SARS-CoV N蛋白的重组逆转录病毒感染显著抑制了人外周血淋巴细胞和其他人细胞系的增殖。

相似文献

2
[Study on interaction between SARS-CoV N and MAP19].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Sep;25(9):777-9.
4
SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity.
Arch Virol. 2012 Apr;157(4):635-45. doi: 10.1007/s00705-011-1221-7. Epub 2012 Jan 6.
7
The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A.
Front Microbiol. 2021 Jun 23;12:551602. doi: 10.3389/fmicb.2021.551602. eCollection 2021.
10
Analysis of multimerization of the SARS coronavirus nucleocapsid protein.
Biochem Biophys Res Commun. 2004 Apr 2;316(2):476-83. doi: 10.1016/j.bbrc.2004.02.074.

引用本文的文献

3
Assessment of genetic diversity, tissue tropism, and antigenic properties of Grimsö betacoronavirus in Swedish bank voles ().
One Health. 2024 Oct 9;19:100911. doi: 10.1016/j.onehlt.2024.100911. eCollection 2024 Dec.
5
Structural proteins of human coronaviruses: what makes them different?
Front Cell Infect Microbiol. 2024 Dec 6;14:1458383. doi: 10.3389/fcimb.2024.1458383. eCollection 2024.
6
Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2.
Int J Mol Sci. 2024 Mar 16;25(6):3378. doi: 10.3390/ijms25063378.
7
SARS-CoV-2 Nucleocapsid Protein Is a Potential Therapeutic Target for Anticoronavirus Drug Discovery.
Microbiol Spectr. 2023 Jun 15;11(3):e0118623. doi: 10.1128/spectrum.01186-23. Epub 2023 May 18.
8
SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions.
Virol J. 2023 Mar 30;20(1):55. doi: 10.1186/s12985-023-02021-2.
9
Molecular mechanisms of human coronavirus NL63 infection and replication.
Virus Res. 2023 Apr 2;327:199078. doi: 10.1016/j.virusres.2023.199078. Epub 2023 Feb 22.
10
Proteomic screening identifies RPLp2 as a specific regulator for the translation of coronavirus.
Int J Biol Macromol. 2023 Mar 1;230:123191. doi: 10.1016/j.ijbiomac.2023.123191. Epub 2023 Jan 9.

本文引用的文献

1
Large-scale preparation and concentration of retrovirus stocks.
Curr Protoc Mol Biol. 2001 May;Chapter 9:Unit9.12. doi: 10.1002/0471142727.mb0912s37.
2
Counting cytokinesis proteins globally and locally in fission yeast.
Science. 2005 Oct 14;310(5746):310-4. doi: 10.1126/science.1113230.
3
Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology.
Nat Struct Mol Biol. 2005 Sep;12(9):772-8. doi: 10.1038/nsmb979. Epub 2005 Aug 21.
5
Effects of severe acute respiratory syndrome (SARS) coronavirus infection on peripheral blood lymphocytes and their subsets.
Int J Infect Dis. 2005 Nov;9(6):323-30. doi: 10.1016/j.ijid.2004.07.014. Epub 2005 Aug 10.
6
Multiple organ infection and the pathogenesis of SARS.
J Exp Med. 2005 Aug 1;202(3):415-24. doi: 10.1084/jem.20050828. Epub 2005 Jul 25.
7
Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein forms a dimer through its C-terminal domain.
J Biol Chem. 2005 Jun 17;280(24):23280-6. doi: 10.1074/jbc.M501015200. Epub 2005 Apr 21.
9
The translation elongation factor 1A in tumorigenesis, signal transduction and apoptosis: review article.
Amino Acids. 2004 Jul;26(4):443-8. doi: 10.1007/s00726-004-0088-2. Epub 2004 Apr 26.
10
Structure of the N-terminal RNA-binding domain of the SARS CoV nucleocapsid protein.
Biochemistry. 2004 May 25;43(20):6059-63. doi: 10.1021/bi036155b.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验