Nunes E V, Campos R, Guilherme C do S, Tolezano J E, Moreira A A, Souza H B, Takiguti C K, Amato Neto V
Instituto Adolfo Lutz, São Paulo, Brasil.
Rev Soc Bras Med Trop. 1991 Oct-Dec;24(4):245-50. doi: 10.1590/s0037-86821991000400007.
We evaluated, comparatively, the performance of female and male Triatoma infestans in the 5th instar of development, in xenodiagnosis for Chagas' disease. Xenodiagnosis were done in 40 patients with chronic infectans. For each diagnosis 20 nymphs female and 20 nymphs males were used, which were weighed immediately before and after the meal. Intestinal contents were examined about 20, 30, 60 and 90 days after the xenodiagnosis application. The females and males ingested the mean of 230 and 210 mg of blood, respectively. The results revealed positivity of 15 (37.5%) out of 40 xenodiagnosis, but any of the positive tests show 100% of the nymphs, female and/or male infected by T. cruzi. The females ingested significantly more blood than the males; this is coincidental with the higher female positivation to T. cruzi here observed. Our results suggest that the female nymphs seem able to increase the sensibility of the xenodiagnosis for chronic patients with Chagas' disease being necessary further studies are necessary to confirm this hypothesis.
我们对发育至五龄期的雌性和雄性克氏锥蝽在查加斯病异嗜性诊断中的表现进行了比较评估。对40例慢性感染者进行了异嗜性诊断。每次诊断使用20只雌性若虫和20只雄性若虫，在进食前后立即称重。在应用异嗜性诊断后约20、30、60和90天检查肠道内容物。雌性和雄性分别平均摄取了230毫克和210毫克血液。结果显示，40次异嗜性诊断中有15次（37.5%）呈阳性，但任何一次阳性检测中，雌性和/或雄性若虫被克氏锥虫感染的比例均未达到100%。雌性摄取的血液明显多于雄性；这与此处观察到的雌性对克氏锥虫的更高阳性率相吻合。我们的结果表明，雌性若虫似乎能够提高对查加斯病慢性患者异嗜性诊断的敏感性，有必要进行进一步研究以证实这一假设。
