贯叶连翘蛋白Hyp-1的结晶及初步晶体学研究，该蛋白与金丝桃素的生物合成有关。
Crystallization and preliminary crystallographic studies of Hyp-1, a St John's wort protein implicated in the biosynthesis of hypericin.
作者信息
Fernandes Humberto, Konieczna Malgorzata, Kolodziejczyk Robert, Bujacz Grzegorz, Sikorski Michal, Jaskolski Mariusz
机构信息
Center for Biocrystallographic Research, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt 5):405-8. doi: 10.1107/S1744309108009111. Epub 2008 Apr 24.
According to a debated hypothesis, the biosynthesis from emodin of the medicinally important natural compound hypericin is catalyzed in St John's wort (Hypericum perforatum) by the phenolic oxidative-coupling protein Hyp-1. Recombinant St John's wort Hyp-1 has been overexpressed in Escherichia coli and obtained in single-crystal form. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit-cell parameters a = 37.5, b = 76.7, c = 119.8 A, contain two protein molecules in the asymmetric unit and diffract X-rays to 1.73 A resolution.
根据一个存在争议的假说，在贯叶连翘（Hypericum perforatum）中，具有重要药用价值的天然化合物金丝桃素由大黄素生物合成是由酚氧化偶联蛋白Hyp-1催化的。重组贯叶连翘Hyp-1已在大肠杆菌中过表达并获得单晶形式。晶体属于正交晶系，空间群为P2(1)2(1)2(1)，晶胞参数a = 37.5，b = 76.7，c = 119.8 Å，不对称单元中含有两个蛋白质分子，X射线衍射分辨率为1.73 Å。
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