Mueller Olaf, Kahmann Regine, Aguilar Guillermo, Trejo-Aguilar Blanca, Wu Andy, de Vries Ronald P
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany.
Fungal Genet Biol. 2008 Aug;45 Suppl 1:S63-70. doi: 10.1016/j.fgb.2008.03.012. Epub 2008 Mar 31.
Ustilago maydis establishes a biotrophic relationship with its host plant, i.e. plant cells stay alive despite massive fungal growth in infected tissue. The genome sequence has revealed that U. maydis is poorly equipped with plant cell wall degrading enzymes and uses novel secreted protein effectors as crucial determinants for biotrophic development. Many of these effector genes are clustered and differentially regulated during plant colonization. In this review, we analyze the secretome of U. maydis by differentiating between secreted enzymes, likely structural proteins of the fungal cell wall (excluding GPI-anchored proteins) as well as likely effectors with either apoplastic or cytoplasmic function. This classification is based on the presence of functional domains, general domain structure and cysteine pattern. In addition, we discuss possible functions of selected protein classes with a special focus on disease development.
玉米黑粉菌与其寄主植物建立了一种活体营养关系，即尽管感染组织中有大量真菌生长，但植物细胞仍保持存活。基因组序列显示，玉米黑粉菌在植物细胞壁降解酶方面的配备不足，而是利用新分泌的蛋白质效应子作为活体营养发育的关键决定因素。许多这些效应子基因在植物定殖过程中聚集且受到差异调控。在本综述中，我们通过区分分泌酶、可能的真菌细胞壁结构蛋白（不包括糖基磷脂酰肌醇锚定蛋白）以及具有质外体或细胞质功能的可能效应子，来分析玉米黑粉菌的分泌蛋白组。这种分类基于功能域的存在、一般结构域结构和半胱氨酸模式。此外，我们讨论了选定蛋白质类别的可能功能，并特别关注疾病发展。
