环磷酸腺苷和多肽生长调节剂在干扰素抑制PC-3细胞生长中的作用。
Role of cyclic AMP and polypeptide growth regulators in growth inhibition by interferon in PC-3 cells.
作者信息
Okutani T, Nishi N, Kagawa Y, Takasuga H, Takenaka I, Usui T, Wada F
机构信息
Department of Urology, Kagawa Medical School Japan.
出版信息
Prostate. 1991;18(1):73-80. doi: 10.1002/pros.2990180107.
Participation of growth factors and intracellular cAMP in direct antiproliferative action of interferon alpha (IFN-alpha) was investigated in PC-3 human prostate carcinoma cell line. IFN-alpha inhibited proliferation of PC-3 cells in a dose-dependent manner in vitro, and the effect was reversible. Fibroblast growth factor, epidermal growth factor and platelet-derived growth factor, when added to the culture medium, showed no effect on growth of PC-3 cells in presence or absence of IFN-alpha. Transforming growth factor beta (TGF-beta) significantly inhibited PC-3 cell growth, and the effect was additived to that of IFN-alpha. TGF-beta content in conditioned medium of PC-3 cells was not affected by treatment with IFN-alpha. On the other hand, IFN-alpha increased intracellular cAMP concentration about 20-fold. Dibutyryl cAMP and reagents which elevated intracellular cAMP level also inhibited PC-3 cell growth. These indicated that direct antiproliferative effect of IFN-alpha on PC-3 cells was at least partly mediated by cAMP, and that neither growth factors nor a growth inhibitor participated in the action of IFN-alpha.
在PC-3人前列腺癌细胞系中研究了生长因子和细胞内cAMP在干扰素α（IFN-α）直接抗增殖作用中的参与情况。IFN-α在体外以剂量依赖的方式抑制PC-3细胞的增殖，且该作用是可逆的。当将成纤维细胞生长因子、表皮生长因子和血小板衍生生长因子添加到培养基中时，无论有无IFN-α，它们对PC-3细胞的生长均无影响。转化生长因子β（TGF-β）显著抑制PC-3细胞生长，且该作用与IFN-α的作用相加。PC-3细胞条件培养基中的TGF-β含量不受IFN-α处理的影响。另一方面，IFN-α使细胞内cAMP浓度增加约20倍。二丁酰cAMP和提高细胞内cAMP水平的试剂也抑制PC-3细胞生长。这些表明IFN-α对PC-3细胞的直接抗增殖作用至少部分由cAMP介导，且生长因子和生长抑制剂均未参与IFN-α的作用。
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