成人眼阵挛-肌阵挛中神经元表面抗原抗体的分析。
Analysis of antibodies to neuronal surface antigens in adult opsoclonus-myoclonus.
作者信息
Sabater L, Xifró X, Saiz A, Alberch J, Graus F
机构信息
Service of Neurology, Hospital Clinic, Universitat de Barcelona, and Institut d' Investigació Biomèdica August Pi i Sunye, Barcelona, Spain.
出版信息
J Neuroimmunol. 2008 May 30;196(1-2):188-91. doi: 10.1016/j.jneuroim.2008.03.013. Epub 2008 May 6.
Antibodies against neuronal surface antigens (NSA-ab) have been described in pediatric opsoclonus-myoclonus syndrome (OMS). We analyzed the presence of NSA-ab by flow cytometry and immunocytochemistry of live cerebellar granular neurons (CGN) in the serum of 25 adult patients with idiopathic (14) and paraneoplastic (11) OMS. Paraneoplastic, but not idiopathic, OMS sera showed a CGN surface binding by flow cytometry higher than that of controls (mean MFI (median fluorescence intensity): 29+/-6.9 vs. 20+/-5.8; p=0.001) but only one serum had a binding greater than three standard deviations of controls. OMS sera did not label live CGN by immunocytochemistry. Unlike pediatric OMS, NSA-ab were not detected in adult cases suggesting that the immunity to NSA in OMS is heterogeneous.
小儿斜视眼阵挛-肌阵挛综合征（OMS）中已发现针对神经元表面抗原的抗体（NSA-ab）。我们通过流式细胞术和免疫细胞化学方法，对25例成年特发性（14例）和副肿瘤性（11例）OMS患者血清中的活小脑颗粒神经元（CGN）进行分析，以检测NSA-ab的存在。副肿瘤性而非特发性OMS患者的血清通过流式细胞术显示出CGN表面结合高于对照组（平均MFI（中位荧光强度）：29±6.9对20±5.8；p = 0.001），但只有一份血清的结合大于对照组的三个标准差。通过免疫细胞化学，OMS血清未标记活CGN。与小儿OMS不同，在成年病例中未检测到NSA-ab，这表明OMS中对NSA的免疫是异质性的。
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