未成熟T细胞中RelB和NFATx的线粒体定位。
The mitochondrial localization of RelB and NFATx in immature T cells.
作者信息
Stasik Izabela, Rapak Andrzej, Zioło Ewa, Strzadała Leon
机构信息
Laboratory of Tumor Molecular Immunobiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, ul. Weigla 12, 53-114, Wrocław, Poland.
出版信息
Cell Mol Biol Lett. 2008;13(4):493-501. doi: 10.2478/s11658-008-0019-4. Epub 2008 May 6.
In order to exert their activity, transcription factors must be transported to the nucleus. Certain transcription factors have also been found on mitochondria. Here, the localization of RelB and NFATx in the mitochondrial fractions of normal thymocytes and thymic lymphoma cells is shown for the first time. CREB was only found in the nucleus, while p50 (NFkappaB) was found in both the nucleus and the cytoplasm, but outside the mitochondria. The translocation of transcription factors to the mitochondria is differentially regulated. Unlike RelB, which is always present in the mitochondrial fraction, NFATx appeared on the mitochondria in cells treated with ionomycin together with an immunosuppressant and inhibitor of calcineurin (FK506). This data reveals that the mitochondrial localization of some transcription factors is precisely controlled by a calcium signal sensitive to FK506 in T cells.
为了发挥其活性，转录因子必须被转运到细胞核。某些转录因子也在线粒体上被发现。在此，首次展示了RelB和NFATx在正常胸腺细胞和胸腺淋巴瘤细胞线粒体组分中的定位。CREB仅在细胞核中被发现，而p50（NFκB）在细胞核和细胞质中均有发现，但不在线粒体中。转录因子向线粒体的转位受到不同的调节。与始终存在于线粒体组分中的RelB不同，NFATx在经离子霉素与免疫抑制剂及钙调神经磷酸酶抑制剂（FK506）处理的细胞的线粒体上出现。该数据表明，某些转录因子的线粒体定位在T细胞中受到对FK506敏感的钙信号的精确控制。
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