Ciofani Gianni, Cascone Maria Grazia, Serino Lorenzo Pio, Lazzeri Luigi
CRIM Lab, Center for Research In Microengineering, Scuola Superiore Sant'Anna, Viale Rinaldo Piaggio, 34-56025 Pontedera (PI), Italy.
J Microencapsul. 2008 Dec;25(8):569-76. doi: 10.1080/02652040802081227.
In this paper a device, based on urease-loaded microspheres, is presented. The first task of this work was the optimization of a procedure for the alginate microspheres realization, having a radius as close as possible to the optimal one necessary to achieve the maximum enzyme exploitation. This optimal radius was calculated theoretically through a mathematical model which describes the concentration of substrate (urea) inside the microspheres on the assumption of a diffusion-reaction mechanism. The enzyme-loaded microspheres were successfully tested in a prototypal device aimed at the depletion of urea from a circulating fluid simulating blood flow: the results showed that urea concentration in the circulating fluid drops down to less than 25% of the initial value after 5 h.
本文介绍了一种基于负载脲酶微球的装置。这项工作的首要任务是优化海藻酸盐微球的制备工艺，使其半径尽可能接近实现酶最大利用率所需的最佳半径。该最佳半径通过一个数学模型进行理论计算，该模型在扩散-反应机制的假设下描述了微球内底物（尿素）的浓度。负载酶的微球在一个旨在从模拟血流的循环流体中去除尿素的原型装置中成功进行了测试：结果表明，循环流体中的尿素浓度在5小时后降至初始值的25%以下。
